alexa Phenotypic And Genotypic Antibiotic Resistance Features Of Staphylococcus Aureus Isolated From Raw Milk And Traditional Cheese
ISSN: 2161-0495

Journal of Clinical Toxicology
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9th Euro-Global Summit on Toxicology and Applied Pharmacology
June 22-24, 2017 Paris, France

Shimaa T Omara, A S Hakim and M A Bakry
National Research Centre, Egypt
Posters & Accepted Abstracts: J Clin Toxicol
DOI: 10.4172/2161-0495-C1-025
Abstract
The objectives of this study were to determine the antibiotic resistance enterotoxigenic S. aureus within 205 raw milk and traditional cheese samples examined. The isolated S. aureus showed resistance, mainly to oxacillin 71 (67.6%, 71/105), penicillin 67 (63.8%, 67/105), erythromycin 47 (44.8%, 47/105), vancomycin 41 (39.1%, 41/105), respectively. The multidrugresistant S. aureus were detected in 54 (51.4%) S. aureus isolates. The isolated S. aureus were screened for the presence of mecA, vanA, and ermC genes; 78 (74.3%, 78/105), 50 (47.6%, 50/105), and 38 (36.2%, 38/105) of the isolated S. aureus strains carried the carried the mecA, ermC, vanA genes respectively. High level of MRSA contamination 52 (49.5%, 52/105) were detected and it was in details as follows; 31 (77.5%, 31/40), 5 (50%, 5/10), 10 (50%, 10/20), 5 (20%, 5/25), and 1(10%, 1/10) within the examined cattle milk, sheep milk, white cheeses, other cheese, and cheddar cheese samples, respectively. 33 (31.4%, 33/105) of the examined S. aureus strains were both phenotypic vancomycin resistant and genotypic vanA gene carrier representing VRSA strains while, 44 (42%, 44/105) of the S. aureus strains were phenotypic erythromycin resistant and genotypic ermC gene carrier. In conclusion, it is evident that, the detection of high level of MRSA contamination 52 (49.5%, 52/105) in milk and cheese samples examined is regarded as a potential public health hazard facing their consumers. It is better to correct the unhygienic behavior during milk production and cheese manufacturing. Regular examination of the milk and traditional cheese for the presence of MRSA is required to provide an indication for the public health hazards associated with their consumption.
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