Phylogenetic Classification And Antibiotic Resistance Study Of Hydrocarbon And Non-hydrocarbon Utilizing Proteobacteria Isolated From Iko River Estuary, Nigeria | 58861
Journal of Medical Microbiology & Diagnosis
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The phylum Proteobacteria is made up of many characterized polar flagellated, Gram-negative, rod-shaped, aerobic bacteria
with the genus Stenotrophomonas and Xanthomonas being accommodated in the family Xanthomonadaceae while the genus
Pseudomonas belong to the family pseudomonadaceae. The 16S rRNA sequences of the 12 hydrocarbon and non-hydrocarbon utilizing
Proteobacteria in this study were determined and matched with available sequences in the ribosomal database project (RDP). Only
one isolate was found to belong to the family Pseudomonadaceae and was of the genus Pseudomonas (Specie: P. aerugonosa DSM
50071 THE978271) while 11 were found to belong to the family Xanthomonadaceae and were of the genus Stenotrophomonas. Among
the 11 Stenotrophomonas, 6 were identified to be Pseudomonas beteli (Stenotrophomonas maltophilia ATCC 19861 TAB021406), 3 as
Pseudomonas hibiscicola and 2 as Stenotrophomonas maltophilia IAM 12423 TAB294553). The blast comparison also showed the
closeness of the genus Stenotrophomonas to the genus Pseudomonas in their 16S rRNA sequence as seen in P. beteli which is known
as S. maltophilia, ATCC 19861. P. beteli, P. hibiscicola and S. maltophilia-IAM 12423 were all species of the genus Stenotrophomonas.
The antibiotic resistance study of all the proteobacteria using gentamicin, ciprofloxacin and clavulin showed that P. Aerugonosa DSM
50071 was resistant to clavulin with a minimum inhibitory concentration of 2000 μg/ml and a minimum bactericidal concentration
of 2000 μg/ml. P. beteli ATCC 19861 and Pseudomonas hibiscicola ATCC 19867 had low level resistance to gentamicin while P.
aeruginosa DSM 50071 and S. maltophilia IAM 12423/S. pavanii ICB 89 were sensitive.
Mmuoegbulam Oluchi Augusta is currently a PhD student of the Department of Microbiology, University of Calabar, Nigeria. She did Molecular Biology Research on the taxonomic classification, plasmid profile and antibiotic resistance of some microbial isolates from Nigeria at CPQBA-UNICAMP, Brazil under TETFUND sponsorship as part of her present PhD program.