alexa Phytochemical Characterization, Anti-inflammatory And In Vitro Anti-arthritic Activity Evaluation Of Hydro-alcoholic Root Extract Of Euphorbia Tirucalli | 14031
ISSN: 2167-0501

Biochemistry & Pharmacology: Open Access
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International Conference on Pharmacognosy, Phytochemistry & Natural Products

Partha Palit, Dhrubojyoti Mukherjee, Poulami Mahanta, Subhash C. Mondal and Tamal Mondal
Accepted Abstracts: Biochem & Pharmacol
DOI: 10.4172/2167-0501.S1.004
Abstract
As per Indian traditional medicinal system, Euphorbia tirucalli is used by the indigenous people of India to cure rheumatism, skin disorders, cough and other ailments. Present study was executed to characterize the roots of Euphorbia tirucalli to screen out the various phytochemicals for their pharmacological evaluation. The extractive value was found to be 8.12% from hydro-alcoholic extraction respectively through maceration process. The preliminary powder analysis was done in UV chamber and chemical treatment in order to characterize the plant. Total ash and moisture content were found to be 16.4% and 0.65% of powder of Euphorbia tirucalli root. Phytochemical analysis shows the presence of steroid and triterpenoid, alkaloid, tannin in hydro-alcoholic extract of Euphorbia tirucalli root (HAEETR). Thin layer chromatography (TLC) was performed with HAEETR and Rf was found as 0.682, 0.707 and 0.768 in n-butanol: acetic acid: water (7:2:1) mobile phase. Root extract demonstrated potentential anti-inflammatory activity showing 85.45% and 98.35% protection from acute inflammation in carrageenan induced rat paw oedema model at 50 and 100 mg/kg dose. In-vitro anti-arthritic models, such as, inhibition of protein denaturation, and proteinase inhibitory actions were studied, with HAR at 250 μg/ml and results showed significant protection compared to standard drug aspirin. Current studies suggest that Euphorbia tirucalli extract could be a promising agent for the treatment of acute inflammation and arthritis, for which further experiments need to be done.
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