alexa Phytochemicals In P. Hysterophorus Have Chemo-preventive Potential To Inhibit The Growth Of HL-60 Cancer Cells Through Cell Cycle Arrest
ISSN: 2167-0501

Biochemistry & Pharmacology: Open Access
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

International Conference on Pharmacognosy, Phytochemistry & Natural Products
October 21-23, 2013 Radisson Blu Plaza Hotel, Hyderabad, India

Shashank Kumar, Gousia Chashoo, Ajit K. Saxena and Abhay K. Pandey
Posters: Biochem & Pharmacol
DOI: 10.4172/2167-0501.S1.003
Abstract
Plants are being used in the treatment of cancer for a long time despite the availability of rich synthetic drugs even today. In the present study in vitro cytotoxic activity of P. hysterophorus L. (Asteraceae), a weed having vigorous growth potential was investigated against four human cancer cell lines viz., PC-3 (prostate), Colo-205 (colon), NCI-H322 (lung) and THP-1 (leukemia) cell lines by using SRB assay. Some extract fractions accounted for 50-98% growth inhibitory activity against different cancer cell lines. The potential cytotoxic extracts in SRB screening were further assayed for its anti tumor activity against HL- 60 (promyelocytic leukemia) cancer cell line at different concentrations (10-100 μg/ml) using MTT assay. Extracts showed dose dependent growth inhibitory activity (up to 80%). Some of the extracts were active only at higher concentrations. For determining the mechanism of cell death in HL-60 cells, cell cycle analysis and mitochondrial membrane potential (ΔΨm) assays were performed. Cell cycle progression was examined using fluorescence activated cell sorting (FACS) analysis. The sub-G1 DNA fraction exhibited about 50% increase in the camptothecin (5 μM) treated HL-60 cells while some P. hysterophorus extract treated cell lines demonstrated about 90% increment. Extracts showed about 20% loss in mitochondrial membrane potential (ΔΨm) in HL-60 cell line indicating activation of apoptotic cascade by potential extracts. Camptothecin (5 μM) was used as positive control which under similar conditions showed about 50% decrease in ΔΨm. P. hysterophorus extracts thus demonstrated chemo- preventive potential by inhibiting the growth of HL-60 cancer cells through cell cycle arrest.
image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]omicsonline.com

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords