alexa Potentiometric Glycerol Biosensor Based On Incorporation Of Glycerol-dehydrogenase On Au Using Layer-by-layer Self-assembled Molecular Wiring Systems
ISSN: 2155-6210

Journal of Biosensors & Bioelectronics
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 
 
In Association with
 
romanian
 
icamb2015

3rd International Conference and Exhibition on Biosensors & Bioelectronics
August 11-13, 2014 Hilton San Antonio Airport, San Antonio, USA

Aishwarya Mahadevan, Duminda A Gunawardena and Sandun Fernando
Accepted Abstracts: J Biosens Bioelectron
DOI: 10.4172/2155-6210.S1.022
Abstract
An intensive interest in glycerol determination exists due to its extensive applications in major industries including medical, food and biofuels. This has led to a quest for a simple, specific, rapid and highly sensitive method for glycerol quantification. This study attempts to develop an enzyme-based electrochemical biosensor for glycerol quantification using NAD+-dependent enzyme, Glycerol dehydrogenase (GlDH) immobilized on a modified gold (Au) electrode. The objective of this study is to fabricate a molecular wiring system to anchor GlDH enzyme onto a Au electrode and to evaluate the efficacy of the proposed wiring system to effectively transport generated charges (as a result of glycerol oxidation) from the enzyme active site to the supporting electrode. A novel concatenation of molecules was utilized to attach the enzyme onto the Au surface via layerby- layer (LBL) self-assembly method. Surface characterization of the enzyme electrodes using cyclic voltammetry and SEM techniques confirmed attachment of enzyme on the Au electrode with the assistance of the tethering molecules. The enzymatic biosensor was assessed for its potentiometric response to glycerol concentrations ranging from low (0.001 M) to very high (10 M). The effects of supporting electrolyte(s) with varying buffers and presence of enzyme stimulators on the electrical potential generated were analyzed and compared. Current sensing-Atomic force microscopy (CS-AFM) was used to elucidate the overall surface electrical conductance of the electrodes. Simple fabrication, high speed of measurement, high sensitivity and possibility of miniaturization are the main advantages of this biosensor.
Biography
Aishwarya Mahadevan is Master?s student majoring in Agricultural & Biological Engineering at Texas A&M University. Her current research is focused on electrochemical biosensor development. She received her BTech in Biotechnology from Sathyabama University, India. Before attending TAMU, she gained professional research experience in Indian Institute of Technology - Madras (IIT-M) as Research Associate for an algae biofuel project.
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords