Reach Us +44-7482-864460
Prediction Analysis Of Transient Receptor Potential Ion Channel And Acetylcholine Receptor Genes In B Lymphocytes From Chronic Fatigue Syndrome/myalgic Encephalomyelitis Patients | 86555
ISSN: 1747-0862

Journal of Molecular and Genetic Medicine
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Share This Page

Prediction analysis of transient receptor potential ion channel and acetylcholine receptor genes in b lymphocytes from chronic fatigue syndrome/myalgic encephalomyelitis patients

Joint Event on 10th International Conference on Genomics and Molecular Biology & 6th International Conference on Integrative Biology

Helene Cabanas, Anne Klein, Samantha Johnston, Donald Staines, Anu Chacko, Thao Nguyen, Emily Knauth and Sonya Marshall Gradisnik

Griffith University, Gold Coast, Australia

Posters & Accepted Abstracts: J Mol Genet Med

DOI: 10.4172/1747-0862-C2-028

Calcium (Ca2+) and acetylcholine (ACh) signaling are important in B cell activation and potential antibody development. The aim of the study was to examine the effects of key genes responsible for these mechanisms from transient receptor potential (TRP) ion channels and acetylcholine receptors (AChRs) in isolated B cells from chronic fatigue syndrome/myalgic encephalomyelitis patients (CFS/ME). Flow cytometric protocols were used to determine B cell purity, followed by singlenuclleotide polymorphisms (SNP) and genotype analysis from 21 TRP ion channel genes and 9 AChR genes examined by iPLEX Gold assay. Exome analysis was conducted using Illumina HiSeq platform and SNP association and genotype was determined using ANOVA (Analysis of Variance) and PLINK analysis. The SNP predictions on splicing events were realized using Automated Splice Site and Exon Definition Analysis server. Eleven CFS/ME patients (mean age 31.8±SD 5.5 years) defined according to the Fukuda criteria and 11 non-fatigued controls (mean age 33.9±5.1 years) were included. Seventy-eight SNPs were associated with CFS/ME: 35 were mAchR M3, the remaining were nAChR delta, nAChR alpha 9, TRPV2, TRPM3, TRPM4, mAChRM2 and mAChRM5. Mutations in the above genes can create or abolish splicing cryptic sites, which could induce important consequences on protein expression. The mutations can also strengthen or weaken binding sites by affecting the affinity with spliceosome elements, consequently inducing an increase or decrease in protein expression. These findings warrant further examination of the above genes in a larger cohort to investigate their potential role in CFS/ME. [email protected]