alexa Preparation And Characterization Of Zein/chitosan/nanohydroxyapatite Nano Composite Scaffold Prepared By Freeze-drying Method For Tissue Engineering
ISSN: 2332-0737

Current Synthetic and Systems Biology
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3rd International Conference on Systems and Synthetic Biology
July 20-21, 2017 Munich, Germany

Abbas Teimouri and Zeinab Shahbazarab
Payame Noor University, Iran
Posters & Accepted Abstracts: Curr Synthetic Sys Biol
DOI: 10.4172/2332-0737-C1-009
Abstract
The nanocomposite scaffolds were synthesized using freeze-drying method by mixing zein, chitosan (CS) and nanohydroxyapatite (nHAp) in the inorganic/organic different weight ratio. The prepared nanocomposite scaffolds were characterized using scanning electron microscopy (SEM), X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FT-IR) and BET studies. Also, degradation, swelling, and biomineralization capability, cell viability and cell attachment of the composite scaffolds were accomplished. Study FTIR spectrum nanocomposite scaffold of zein/chitosan/nano hydroxyapatite (zein/cs/nHAP) confirms the presence of nano-hydroxyapatite in the polymer field. The study tests SEM, XRD, SEM and BET can be concluded that zein/chitosan (zein/ cs) scaffold cannot be changed in the presence of nano-hydrpxyapatite and micro-pores in the scaffold with good communication and influence to facilitate cell migration and there is also supplying the cell. The biological properties of the scaffolds affected nHAp content changing the ratio of CS in the scaffolds. The test thermal analysis (TGA) indicated a slower destruction zein/chitosan/nanohydroxyapatite nano composite (40/40/20) compared with two other polymers scaffolds. Bio-ceramic nano-hydroxyapatite increase in scaffolding zein/chitosan (zein/cs) increases the compressive strength of the scaffold, but decreases the amount of water absorption and porosity and speed of degradation of scaffolds due to the nano particles hydroxyapatite of pores in the scaffold of zein/chitosan (zein/cs). The biological answer of MG-63 cells on nanocomposite scaffolds was exceptional regarding enhanced cell attachment, higher proliferation, and expansion. The results associated with physicochemical properties and superior cyto-compatibility suggests that zein/cs/nano hydroxyapatite scaffold are potential candidate materials for tissue engineering.
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