alexa Preparation And Evaluation Of Inclusion Complexes Of Papain With HP-β-CD
ISSN: 2167-0501

Biochemistry & Pharmacology: Open Access
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

International Conference on Pharmacognosy, Phytochemistry & Natural Products
October 21-23, 2013 Radisson Blu Plaza Hotel, Hyderabad, India

A. Swapna, C. V. S. Subrahmanyam and M. Lakshmi Narasu
ScientificTracks Abstracts: Biochem & Pharmacol
DOI: 10.4172/2167-0501.S1.002
Abstract
Inclusion complexes of β-cyclodextrin are useful to improve solubility and stability of drugs and therapeutic proteins. Protein molecule actives were incorporated in to HP- β-CD for improving the dissolution, bioavailability. Studies permit to understand their properties and possible therapeutic applications. Papain (digestive enzyme) was chosen and the factors, namely substrate concentration, pH of the medium, and temperature influencing enzyme activity were investigated. A suitable analytical method was established for papain at λ max 660 nm. It was verified that there is no interference of hydroxy propyl β-cyclodextrin on the analytical wavelength of enzyme. Enzymatic activity of papain was assayed at increasing concentrations of hydroxy propyl β-cyclodextrin. The enzyme activity was decreased as the concentration of HP-β-CD increased, indicating inaccessibility of papain to the substrate and also increasing complexation. There was no shift in optimum pH 7.5 on adding HP-β-CD. Optimum temperature for papain was 30?C and temperature stability was achieved till 55?C, in presence of HP-β-CD. Complexes of different ratios were prepared from 1:10 to 1:200 by physical mixing, solvent evaporation and neutralization and assayed. By physical mixtures, the enzymatic activity was found to be enhanced compared to papain alone and optimum for 1:40. The free activity was reduced gradually in alkaline pH, but inclusion complex preserved its activity even in alkaline pH, and extreme temperatures. Inclusion complexes were estimated by IR and shown the presence of enzyme and HP-β-CD in the complex?s bands. Keywords: Enzyme, Papain, Hydroxy Propyl β- Cyclodextrin, Enzyme activity, Inclusion complexes
image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

agrifoodaquavet@omicsonline.com

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

clinical_biochem@omicsonline.com

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

business@omicsonline.com

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

chemicaleng_chemistry@omicsonline.com

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

environmentalsci@omicsonline.com

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

engineering@omicsonline.com

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

generalsci_healthcare@omicsonline.com

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

genetics_molbio@omicsonline.com

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

immuno_microbio@omicsonline.com

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

omics@omicsonline.com

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

materialsci@omicsonline.com

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

mathematics_physics@omicsonline.com

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

medical@omicsonline.com

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

neuro_psychology@omicsonline.com

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

pharma@omicsonline.com

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

social_politicalsci@omicsonline.com

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords