Preparation of BSA nanoparticles by desolvation technique using ethyl alcohol as desolvating agent

2^nd International Conference and Exhibition on Pharmaceutical Regulatory Affairs

November 23-24, 2012 Hyderabad International Convention Centre, India

A.Krishna Sailaja and P. Amareshwar

Posters: Pharmaceut Reg Affairs

Abstract :

In order to see functionality and toxicity of nanoparticles in various food and drug applications, it is important to establish procedures to prepare nanoparticles of a controlled size. Desolvation, a thermodynamically driven self-assembly process for polymeric materials.Here BSA nanoparticles were prepared by desolvation technique using Ethyl alcohol as desolvating agent. In our study, Ethyl alcohol was added intermittently into 1% BSA solution under stirring at 700 rpm. Amount of Ethanol added, intermittent timeline of Ethanol addition, and pH of solution were considered as process parameters to be optimized. Effect of the process parameters on size of the nanoparticles were studied. The results indicated that the size control of BSA nanoparticle was achieved by adding Ethanol intermittently. The standard deviation of average size of BSA nanoparticles at each preparation condition was minimized by adding Ethanol intermittently. Particle size of BSA nanoparticles prepared by continuous addition of Ethyl alcohol as desolvating agent was in between 200-300nm.Whereas uniform particle size was obtained by adding Ethyl alcohol intermittently. The particle size was found to be in between 180-220nm.FTIR spectra Indicates that there was no drug and polymer interaction.

Biography :

A.krishna sailaja is working as senior research scholar pursuing Ph.D in Osmania University Hyderabad in Pharmacy Department. She has published 13 papers in various national and international Journals. She has presented papers in One national and One International conferences. Previously she worked as Assistant Professor in Mallareddy college of Pharmacy and Mother Teresa college of pharmacy for a period of three years. Worked as Drugs Inspector in Drugs control administration Hyderabad for a period of 1 year. Worked as JRF and working as SRF in Osmania University