Probiotics are defined as live microorganisms that, when administered in adequate amounts, confer a health benefits on the host.
The global and domestic probiotic market is expected to be more than US$ 32.6 billion and about Rs.20.6 million respectively by
2015. The probiotic enriched foods are the attractive area of research, due to their preventive and sometimes curative effects against
various diseases. The probiotic potential of Lactobacillus rhamnosus RSI3, a strain isolated from a traditional cereal-based fermented milk
product (Raabadi) by in-vitro method taking L. rhamnosus GG (LGG) as a control was evaluated. The in-vitro tests included acid and bile
tolerance, bile salt deconjugation, cell surface hydrophobicity, antimicrobial, antifungal and beta galactosidase activity and sensitivity to
antibiotics. During acid tolerance RSI3 had shown good survivability in acidic condition at pH 2.0 and 3.0, bile salt tolerance upto 0.5%,
Cell surface hydrophobicity in different hydrocarbons such as n-Hexadecane 19% and n-Octane 20%. RSI3 shown sensitive to various
antibiotics but it has shown resistant to vancomycin which is a typical characteristic of LAB for potential probiotics. RSI3 has shown
discernible antibacterial activity against various pathogenic and spoilage causing organisms such as E. coli, Salmonella typhimurium,
Listeria monocytogens and Staphylococcus aureous and strong antifungal activity against various yeasts and moulds such as Kluveromyces
marxianus 46, Candida butyri 280, Aspergillus flavus 268 and Sacchaomyces cerevisiae. RSI3 showed a good β-galactosidase activity
indicative by colour change within 1-4 hour capability to utilize lactose.
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