Biochemistry & Pharmacology: Open Access
Open Access
ISSN: 2167-0501
+44-20-4587-4809
ISSN: 2167-0501
+44-20-4587-4809
Aloisio Ferreira Pinto Neto
Accepted Abstracts: Biochem & Pharmacol
Objective: To evaluate the effects of aqueous extract of Hyptis fructicosa in liver regeneration after partial hepatectomy in rats. Materials and methods : The study was conducted in 16 Wistar rats (300 to 450 g). All animals were randomly divided into two groups, which consisted of eight animals each: control group (CG), the rats received water for 4 days and the HF group, where rats received aqueous extract of Hyptis fructicosa for 4 days at a dose of 100 mg / kg / day. In this consecutive day treatment, the animals of both groups were submitted to partial hepatectomy approximately 67% of the liver. All operations were performed under inhalation anesthesia with ether. After 24 hours, they underwent a new operation to remove the liver remanascente. Then they were sacrificed. Liver regeneration was evaluated by PCNA immunohistochemistry using monoclonal anti-PCNA primer (PC-10; DAKO A / S, Glostrup, Denmark). All data are expressed as mean � standard deviation. Statistical comparisons between groups were performed by Mann-Whitney test (p<0.05). Results : The rate of liver regeneration, which was assessed by proliferating cell nuclear antigen (PCNA), using monoclonal anti-PCNA primary, was significantly higher on the HF group than on the control group (CG). On the HF group this rate was 53.56%�18.91 while the CG presented index 21.12�8.29%. With the use of aqueous extract of Hyptis fructicosa , liver regeneration increased by about 2.5 times. Conclusion : The present study shows that the aqueous extract of Hyptis fructicosa dose of 100 mg/kg/day stimulated liver regeneration in rats.
Aloisio Ferreira Pinto Neto has completed his graduation at the age of 26 years from the Universidade Federal de Sergipe, one of the ten best medical universities of the country.