alexa Properties Of Ghost Bacterial Cells Obtained By Hybrid Material With Silver Nanoparticles (PVA/AgNps) As Well As Their Opportunities For Use In Vaccine Prophylactics
ISSN: 2157-7560

Journal of Vaccines & Vaccination
Open Access

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4th International Conference on Vaccines & Vaccination
September 24-26, 2014 Valencia Convention Centre, Spain

Daniela Pencheva
ScientificTracks Abstracts: J Vaccines Vaccin
DOI: 10.4172/2157-7560.S1.020
Abstract
P VA/AgNps is hybrid material with thermally reduced silver nanoparticles, stabilized in polyvinyl-alcohol. It is well characterized with experimental results from physico-chemical, microbiological and cytological tests. In vivo experiments in dermal cytotoxicity test and subcutaneous injections on white mouse showed PVA/AgNps as a non-toxic in the enclosed silver concentration. It was conducted an experiment to implement it as an inactivator of a bacterial strain E. coli O 104 for the preparation of antigen for immunization of rabbits. It has been used also for preservative of the obtained in consequence of immunization hyperimmune E. coli O104 rabbit antiserum. It has been successfully used in clinical trials as a treatment agent for cough and recurrent otitis in dogs. The activity of PVA / AgNps was tested to nearly 150 bacterial and fungal strains. The MBC of synthesized samples of the hybrid material are determined also for E. coli O 149, E. coli O 157 H7 and S. Typhimurium, which are established as common pathogens in farm animals with huge losses for animal farming. As a result of all the in vitro and in vivo tests, the hybrid material was characterized as a non-toxic product with a bactericidal and fungicidal action to control and clinical strains of bacteria and yeast in the established tests limits, with excellent prospects to be used as a preservative for diagnostic serums and as an inactivator for obtaining of ghost cells with capacity for application as whole cell killed vaccines, and theoretical perspectives in the preparation of recombinant vaccines.
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