In thepresentstudy, proteomic analysis was used as a tool to help understanding the molecular mechanisms underlying chronic
fluoride toxicity in rat liver. Three groups of weanling male Wistarrats (21 days) were treated with drinking water containing 0
(control), 5 or 50 mg/L fluoride for 60 days (n=6/group). After euthanasia, liver and serum were collected. The left hepatic lobe
and plasma were analyzes for fluoride using the ion-sensitive electrode, after hexamethyldisiloxane-facilitated diffusion. Data
were analyzed by ANOVA and Tukey?s test, (p<0.05). The median hepatic lobe was used for proteomic analysis by 2D-PAGE
associated with LC-MS/MS. Gels were digitalized using ImageScaner III (GE healthcar) and ANOVA statistical analysis was used
to compare the differences in intensity of each matched protein spot. The p-values were calculated using the Image Master 2D
Platinum v7.0 software (GE Healthcare). The spots presenting significant difference (p<0.05) were excised and identified by LCMS/
MS. From the differentially expressed spots, 94 proteins were satisfactorily identified (72.3%) and divided into 5 functional
categories. Most of the proteins were related to ?metabolism and energy? (52%), followed by ?information pathways? (18%), ?cell
processes? (12%), ?structure and cell organization? (11%) and ?transport? (7%). Based on the present results, it can be concluded
that fluoride alters the expression of several hepatic proteins belonging to all the functional categories, especially those related to
metabolism, and these alterations are more evident for the group treated with 50 mg/L fluoride.
Aline de Lima Leite is graduated in Biological Sciences from Universidadedo Sagrado Cora??o(USC), Bauru, Brazil.She has completed her M.Sc.
in Oral Biology from Bauru Dental School, University of S?o Paulo (USP), and nowadays is Ph.D student in Evolutionary Genetics and Molecular
Biology at Federal University of S?o Carlos (UFSCar), S?o Carlos, Brazil. Leitehas 11 published papers and 1 book chapter.
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