alexa
Reach Us +441414719275
Proteomic Analysis Of Milk From Greek Indigenous Capra Prisca Goat Breed: Methodology Development And Initial Results | 8853
ISSN: 2157-7579

Journal of Veterinary Science & Technology
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Proteomic analysis of milk from greek indigenous capra prisca goat breed: Methodology development and initial results

International Conference on Animal & Dairy Sciences

G.C. Fthenakis, A.K. Anagnostopoulos, A.I. Katsafadou, A. Arsenos, I. Skoufos4, A. Tzora, S. Karkabounas and G. Tsangaris

Posters: J Veterinar Sci Technolo

DOI: 10.4172/2157-7579.S1.003

Abstract
This paper presents development of methodology for proteomic analysis of milk samples of Greek goats and initial findings of the work. 10 ml of milk were collected in sterile vials and stored at -80 o C. Each sample was fragmented in three layers (lipid-, casein-, whey-) by centrifugation at 14,000 rpm for 1 h. 2-DE was performed in total milk, in casein-layer and in whey-layer. Protein content of each sample was measured colorimetrically by the Bradford assay; 1 mg of total protein was diluted in a sample buffer containing urea, thiourea, Chaps. For the first dimension, IPG strips of 3-10NL pI and 4-7L were used and the samples were electrophoresed at 6,000 V for 152,000 Vh. Subsequently, the second dimension was performed on 12% SDS-polyacrylamide gels, which were further stained overnight with colloidal coomassie blue. The method was performed successfully in five samples. Total number of spots detected was 822 (163 ? 17 spots per gel). Analysis by MALDI ToF-MS resulted in identification of 285 single-gene products in total. This is the first description of research presenting proteomic evaluation of milk from indigenous Greek goats. Successful development of methodology will support detailed studies into proteomic evaluation. Description of proteomic characteristics of the breed will support construction of dataset for genetic characterization. Work in this presentation has received funding from the ?GOSHOMICS? program, which takes place within the SYNERGASIA 2009 action and is supported by the European Regional Development Fund and Greek national funds, project number 09SYN-23-990. The text represents the author?s? views.
Biography
G.C. Fthenakis, DVM, M.Sc., PhD, DipECAR, DipECSRHM is Professor of Veterinary Reproduction at the Veterinary Faculty of the University of Thessaly, Greece, Past-President of the European College of Small Ruminant Health Management and Fellow of the Greek Agricultural Academy. He has published 122 refereed papers (total impact factor: 167.532), which have received >1650 citations. He has managed many research grants, funded from the public (European Commission, Greek Ministry of Education) or the private (animal health industry) sector.
Top