This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.
This paper presents development of methodology for proteomic analysis of milk samples of Greek goats and initial findings of
the work. 10 ml of milk were collected in sterile vials and stored at -80
C. Each sample was fragmented in three layers (lipid-,
casein-, whey-) by centrifugation at 14,000 rpm for 1 h. 2-DE was performed in total milk, in casein-layer and in whey-layer.
Protein content of each sample was measured colorimetrically by the Bradford assay; 1 mg of total protein was diluted in a sample
buffer containing urea, thiourea, Chaps. For the first dimension, IPG strips of 3-10NL pI and 4-7L were used and the samples
were electrophoresed at 6,000 V for 152,000 Vh. Subsequently, the second dimension was performed on 12% SDS-polyacrylamide
gels, which were further stained overnight with colloidal coomassie blue. The method was performed successfully in five samples.
Total number of spots detected was 822 (163 ? 17 spots per gel). Analysis by MALDI ToF-MS resulted in identification of 285
single-gene products in total. This is the first description of research presenting proteomic evaluation of milk from indigenous
Greek goats. Successful development of methodology will support detailed studies into proteomic evaluation. Description of
proteomic characteristics of the breed will support construction of dataset for genetic characterization. Work in this presentation
has received funding from the ?GOSHOMICS? program, which takes place within the SYNERGASIA 2009 action and is supported
by the European Regional Development Fund and Greek national funds, project number 09SYN-23-990. The text represents the
G.C. Fthenakis, DVM, M.Sc., PhD, DipECAR, DipECSRHM is Professor of Veterinary Reproduction at the Veterinary Faculty of the University of
Thessaly, Greece, Past-President of the European College of Small Ruminant Health Management and Fellow of the Greek Agricultural Academy.
He has published 122 refereed papers (total impact factor: 167.532), which have received >1650 citations. He has managed many research grants,
funded from the public (European Commission, Greek Ministry of Education) or the private (animal health industry) sector.
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals