Objective: The aim of this study was to use proteomic techniques to identify the proteins in squamous cervical cancer whose
expression levels changes in response to neoadjuvant chemotherapy (NAC).
Methods: We used bulky squamous cervical cancer tissues (16 cases) before and after NAC treatment from patients who responded
to NAC. Six cases before and after NAC treatment were selected for the two-dimensional gel electrophoresis, and the differentially
expressed proteins were identified using matrix-assisted laser desorption/ionization-time of flight mass spectrometry. The other
10 pairs of tissues were selected to confirm the expression and localization of the peroxiredoxin-1 and galectin-1 proteins using
western blot analysis and immunohistochemistry.
Results: The comparison of the proteins present before and after NAC revealed that 116 protein spots were up-regulated or
down-regulated. Thirty-one of these proteins were analyzed by mass spectrometry, and 15 proteins were up-regulated in the
cancer tissue after NAC relative to the level before NAC, whereas 16 proteins were down-regulated after NAC. The expression of
peroxiredoxin-1 was significantly higher and the expression of galectin-1 was significantly lower after NAC treatment than before
Conclusions: Proteomics can be used to identify chemotherapy-related proteins in squamous cervical cancer. The up-regulation
of peroxiredoxin-1 and the down-regulation of galectin-1 may play distinctive roles in the response of squamous cervical cancer
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