The development of epilepsy is often associated with marked changes in central nervous system cell structure and function.
Along these lines, reactive gliosis and granule cell axonal sprouting within the dentate gyrus of the hippocampus are
commonly observed in individuals with temporal lobe epilepsy. Here we used the pilocarpine model of temporal lobe epilepsy
in rats to screen the proteome of the neurons situated in the granular cell layer on the dentate gyrus to identify molecular events
that are altered as part of the pathogenic process. Using laser microdissection and two-dimensional gel electrophoresis-based
approach, followed by mass spectrometry, 39 differentially expressed proteins were identified. Functionally, these proteins were
organized into several classes, including synaptic physiology, cell structure, cell stress, metabolism and energetics.Together, these
data provide new molecular insights into the altered protein profile of the epileptogenic dentate gyrus and can contribute to a
better understand of phenomena involved in the genesis and maintenance of the epileptic state. In addition, such proteins may
be used as a specific marker to the early events of epileptogenesis, and possibly be postulated as interesting therapeutic targets for
the control of epileptic seizures.
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