alexa Proximity Ligation Assays, A Method For Ultra Sensitive Protein Detection
ISSN: 2157-7633

Journal of Stem Cell Research & Therapy
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading Please wait..

3rd International Conference and Exhibition on Cell & Gene Therapy
October 27-29, 2014 Embassy Suites Las Vegas, USA

Stephen Lin
ScientificTracks Abstracts: J Stem Cell Res Ther
DOI: 10.4172/2157-7633.S1.007
Quantitative PCR (qPCR) has revolutionized the study of cellular nucleic acids, including genomic DNA, mRNA and microRNAs. Now TaqMan? Protein Assays, which are based on the proximity ligation assay (PLATM) technology, extend qPCR applications to the analysis of proteins through the amplification of a surrogate DNA template. This technology has been optimized for use with crude cell lysates, tissue homogenates, and blood samples and has been combined with TaqMan? chemistry to create a highly sensitive and specific process for measuring proteins. With its simple and rapid workflow akin to traditional qPCR techniques, PLA enables a broad range of applications that includes the analysis of proteins from complex or difficult samples such as serum and plasma, protein modifications such as phosphorylation, and protein and gene expression from a single sample and even a single cell in parallel on the same platform. Moreover, PLA is being applied in new ways to study normal and aberrant biological processes that are associated with homeostasis, growth and differentiation. The addition of TaqMan? Protein Assays to the qPCR assay repertoire marks the beginning of a new era in the analysis of proteins that will ultimately provide a better understanding of biological systems.
image PDF   |   image HTML
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version