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Quantitation Of Alpha Amyrin In Scoparia Dulcis L. Whole Plant Extract By High Performance Liquid Chromatography | 8353
ISSN: 0975-0851

Journal of Bioequivalence & Bioavailability
Open Access

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Quantitation of Alpha Amyrin in Scoparia dulcis L. Whole Plant Extract by High Performance Liquid Chromatography

International Conference & Exhibition Bioequivalence and Bioavailability

Lakshmu Naidu. P. V, Kishore Kumar. K, Sujatha. S, Narasimha Rao. M

ScientificTracks Abstracts: J Bioequiv Availab

DOI: 10.4172/0975-0851.1000001

Abstract
A rapid and sensitive HPLC method with UV detection (206 nm) for routine analysis of alpha amyrin from Scoparia dulcis plant extract was developed. Chromatography was performed with mobile phase containing a mixture of acetonitrile and water (59:41) with a flow rate of 1.5 mL min -1 . The procedure was validated by linearity (correlation coefficient = 0.9997), accuracy, robustness and intermediate precision. LOQ and LOD of detection were found to be 1.2 μg and 0.4 μg respectively. Linearity established at different concentrations of alpha amyrin ranging from 0.008mg mL -1 to 0.032 mg mL -1 . The proposed method was simple, highly sensitive, precise, accurate and repeatable.
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