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pider silk has extreme mechanical properties, is well tolerated when implanted in living tissue and has been shown to enable
regeneration of peripheral nerves. The miniature spider silk protein 4RepCT can be processed into solid films, fibres and
foams. The material is of non-animal origin since it is produced in E. coli and is well tolerated when implanted subcutaneously
in rats. Furthermore, 4RepCT can easily be fused to cell binding peptides and thereby a customized matrix can be produced.
4RepCT matrices support the proliferation of undifferentiated rat neural stem cells, and the cells can then successfully be
differentiated into astrocytes, neurons or oligodendrocytes. Current work is focused on 1) enabling culture and studies of neural
stem cells in three-dimensional environments, and 2) to culture human embryonic stem cells and induced pluripotent stem
cells on the spider silk matrices. The possibility to make implantable three dimensional, mechanically robust structures of the
recombinant spider silk proteins, in combination with the option to functionalize the material with cell adhesion peptides make
recombinant spider silk a unique material for cell culture and tissue engineering.
Anna Rising is a veterinarian and completed her Ph.D. at the age of 31 years from the Swedish University of Agricultural Sciences (SLU) and
currently holds positions as an Assistant Professor at Karolinska Institutet and as Lecturer in Translational Veterinary Medicine at SLU. She is co-
founder of Spiber Technologies AB, a spin-off company producing recombinant spider silk. She has published more than 20 papers in international
peer reviewed journals.
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