Metabolism transforms compounds to produce energy and building blocks. Metabolic network reconstruction allows better
understanding of the metabolism and the catabolism of living cells and is commonly available at the metabolite level.
Atom-level representation of the pathways could yield many insights into the exact mechanisms of enzymatic reactions. The
ability to trace the fate of individual atoms through the metabolic pathways will also be very useful in many applications of
metabolic engineering, systems biology and drug discovery. So far, the atom mappings in enzymatic reactions have not been
studied in depth and most of the metabolic databases do not contain comprehensive information on atom tracing through
database contains such information between single metabolite pairs; however, it would be
difficult to extend these mappings for every pathway and in every organism.We developed a computational framework for the
atom labeling and tracking through metabolic pathways. This framework analyzes all atom transitions based on enzymatic
reaction rules by tracking the movement of every single atom through metabolic pathways. Our method allows a straightforward
and computationally efficient means for the observation of all possible fluxes in a proposed network model based on atom
We demonstrate the usefulness of our framework through a case study of biosynthesis of 3-Hydroxypropanoate (3HP) in
and we compare the efficiency of different pathways in terms of the fate of the labeled atoms of substrates.
Noushin Hadadi is currently a Ph.D. student and research assistant in the group of Professor Vassily Hatzimanikatis at the Laboratory of Computational
Systems Biotechnology (LCSB) at EPFL. Noushin earned her B.Sc. in Chemical Engineering at University of Tehran, Iran, in 2007. In 2008, Noushin
joined EPFL as a Master student. She received her Master?s degree in Biochemical Engineering in 2011, and started her Ph.D. right afterwards. Her
research interests include the development of computational frameworks for better understanding the metabolic networks of model organisms such
Having full description of enzymatic reactions in metabolic networks helps for the discovery of novel metabolic pathways for the microbial
production of valuable chemicals.
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