This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.
Excessive scar production after myocardial infarction (MI) seriously compromises cellular regeneration. This study explores the
role of collagen deposition on engraftment of progenitor cells in the myocardium.
A tri-cell patch (Tri-P) consisting
of iPSC derived cardiomyocytes (CM), endothelial cells (EC), and mouse embryonic fibroblasts (MEF) was cultured and seeded
on isolated peritoneum. The expression of fibrosis related molecules from MEF and the infarcted heart was measured by Western
blot and qPCR.
The Tri-P was overlaid on the entire infarcted area at 7 days after MI in overexpressing adenylyl cyclase
VI (AC6) and WT mice. A combination of
bioluminescent imaging (BLI) and postmortem
analysis, as well as
counting the number of green fluorescent protein positive cells (GFP
) was used to assess engraftment efficiency of progenitor
cells. Echocardiography was performed weekly. Hearts were harvested for analysis 4 weeks after Tri-P application. MEF were
stimulated with forskolin before an anoxia/reoxygenation protocol.
Several fibrosis related molecules
were analyzed. In AC6 mice,
infarcted hearts treated with Tri-P showed significantly higher BLI signals and a higher number of GFP
cells as compared with
WT mice. Heart function, which progressively improved from week 2 to week 4, was associated with reduced left ventricular
fibrosis in AC6 mice as compared to WT mice after MI. The application of a Tri-P in AC6 mice with decreased collagen and
sparsely distributed connective tissue showed significantly higher iPSC engraftment and subsequent angiomyogenesis in an
Yigang Wang has completed his medical degree in 1983, PH. D in1997 and have been in academic research ever since. He is an associate
professor in the Department of Pathology and Laboratory Medicine, University of Cincinnati. He has served as an editorial board member of several
journals in the U.S. He has published more than 50 papers in reputed journals. Over the past 2 decades, his research focus has been in three areas:
1) Protection of the ischemic myocardium testing cardioplegic solutions for cardiac ischemic injury and their effect on hemodynamic changes; 2)
Ischemic preconditioning against ischemia/reperfusion injury, its molecular mechanisms, and signaling pathways; 3) Progenitor cell based therapy
for treatment of myocardial infarction.
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals