alexa Relationship Between Anesthetic-induced Toxicity And NMDA Receptor-mediated Calcium Influx In Developing Neurons
ISSN: 2157-7013

Journal of Cell Science & Therapy
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading Please wait..

8th World Congress and Expo on Cell & Stem Cell Research
March 20-22, 2017 Orlando, USA

Cheng Wang, Fang Liu, Tucker A Patterson, Merle G Paule and William Slikker
National Center for Toxicological Research/Food and Drug Administration, USA
Posters & Accepted Abstracts: J Cell Sci Ther
DOI: 10.4172/2157-7013.C1.039
Ketamine is a non-competitive NMDA receptor antagonist and is used as a general anesthetic. Recent data suggest that anesthetics can cause neuronal damage when exposure occurs during development. To elucidate the underlying mechanisms associated with ketamine neurotoxicity, neural cells were harvested from the forebrain of newborn rats and neural stem cells were isolated from gestational day-16 rats. To determine the effect of ketamine on developing neurons and undifferentiated neural stem cells, cultures were exposed to 10 μM ketamine for 24 hours. Ketamine exposure resulted in elevated NMDA receptor (NR1) expression in primary cultures, and enhanced damage of developing neurons including those differentiated from the neural stem cells. However, the viability and proliferation rate of neural stem cells were not significantly affected after ketamine exposure. Calcium imaging data indicated that 50 μM NMDA did not cause a significant influx of calcium in typical neural stem cells; however, it has produced an immediate elevation of intracellular free Ca2+ [Ca2+]i in neurons differentiated from the same neural stem cells. These findings suggest that prolonged exposure of developing neurons to ketamine produces an increase in NMDA receptor expression, which allows for a higher/toxic influx of calcium into neurons once ketamine is removed from the system, leading to neuronal cell death likely due to elevated reactive oxygen species generation.

Email: [email protected]

image PDF   |   image HTML

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version