Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Mar�lia Afonso Rabelo Buzalaf, Juliane Guimar�es Carvalho, Aline de Lima Leite, Camila Peres-Buzalaf, Eric Everett and Gary Milton Whitford
Scientific Tracks Abstracts: J Proteomics Bioinform
A/J and 129P3/J mouse strains have different susceptibilities to dental fluorosis (DF) due to their genetic backgrounds. They also differ with respect to several features of fluoride (F) metabolism and metabolic handling of water. This study was done to determine whether differences in F metabolism could be explained by diversities in the profile of protein expression in kidneys. Weanling, male A/J mice (susceptible to DF, n=18) and 129P3/J mice (resistant, n=18) were housed in pairs and assigned to three groups given low-F food and water containing 0, 10 or 50 ppmF for 7 weeks. Renal proteome profiles were examined using 2D-PAGE and LC-MS/MS. Quantitative intensity analysis detected between strains A/J and 129P3/J 122, 126 and 134 spots differentially expressed in the groups receiving 0, 10 and 50 ppmF, respectively. From these, 25, 30 and 32, respectively, were successfully identified. Most of the proteins were related to metabolic and cellular processes, followed by response to stimulus, development and regulation of cellular processes. In F-treated groups, proteins involved in the regulation of renal tubular reabsorption capacity were down-modulated in kidney from 129P3/J mice. A/J and 129P3/J mice exhibited 13 and 4 exclusive proteins, respectively, regardless exposure to F. In conclusion, proteomic analysis was able to identify proteins potentially involved in metabolic handling of F and water that are differentially expressed or even not expressed in the strains evaluated. This can contribute to understanding the molecular mechanisms underlying genetic susceptibility to DF, by indicating key-proteins that should be better addressed in future studies.
Mar�lia Buzalaf is graduated in Dentistry from University of Sao Paulo (USP), Bauru, Brazil. She has completed her PhD, postdoctoral and since 2007 she is Chair Professor of Biochemistry and Cariology at the Department of Biological Sciences at USP, Bauru. Recently, Dr. Buzalaf has focused her interest toward the areas of Cariology, dental fluorosis and Proteomics. Nowadays, she mentors 25 students, including undergraduate, graduate and post-doctoral students. In addition, she has published more than 200 papers in high impact journals and is author of 2 scientific books. As an educator, Dr. Buzalaf expresses the same enthusiasm in sharing her expertise with students in general and oral biochemistry.