alexa Report On The Phylogenetic Studies Of Diplostomatid Parasites | 63477
ISSN: 1745-7580

Immunome Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

8th Molecular Immunology and Immunogenetics Congress

Mitonga M M A, Moema E B E, King P H and Rakgole J N
Sefako Makgatho Health Sciences University, South Africa
Posters & Accepted Abstracts: J Immunome Res
DOI: 10.4172/1745-7580.C1.012
Abstract
Introduction: Diplostomid metacercariae inhabit freshwater fish species as the second intermediate hosts. These parasites have been found in the eye lens, the retina, vitreous humor and the nervous system of freshwater fish. The classification of these parasitic stages to the species level using only morphology is often difficult and ambiguous. The use of molecular techniques has allowed links to be elucidated using various developmental stages of these parasites. The aim of this study was to provide a summative report on the phylogenetic tree by applying molecular biology techniques to the investigation of larval diplostomid parasites. Materials & Methods: Diplostomid metacercariae were preserved in 70% ethanol prior to DNA extractions using Qiagen kit. Standard techniques for amplification of rRNA region were followed. The DNA amplicons were sent to Inqaba Biotech laboratory for sequencing and phylogenetic trees generated using software programs. Results: The amplicons of these diplostomids had band sizes of 500 base pairs. The amplicons contained only partial regions (ITS-2). The parasitic species 28S rDNA genomic region was successfully amplified. Conclusion: The application of molecular techniques on digenetic trematodes seems very promising and may yield great potential in future descriptions of morphologically similar parasitic species.
Biography

Mitonga M M A received his Bachelor of Science degree from the University of Limpopo, Medunsa Campus, Pretoria, in South Africa. In 2013, he joined the Department of Biology, Sefako Makgatho Health Sciences University as a Senior Technical Officer. Currently, his project focuses on the morphology and molecular characterization of metacercariae from freshwater fish of South Africa.

Email: [email protected]

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7