alexa Role Of Exosomes In Pericardial Fluid-mediated Cardiac Regeneration | 17448
ISSN: 2157-7013

Journal of Cell Science & Therapy
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

4th World Congress on Cell Science & Stem Cell Research
June 24-26, 2014 Valencia Conference Centre, Valencia, Spain

Federica Limana
Accepted Abstracts: J Cell Sci Ther
DOI: 10.4172/2157-7013.S1.030
Abstract
Background: We recently demonstrated that epicardial stem cells participate to the regenerative response following myocardial infarction (MI) and factors released in the pericardial fluid (PF) may play a key role in this process. Exosomes are secreted microvescicles of endocytic origin, identified in most body fluids, which may contain proteins able to modulate cell functions. Here, we investigated whether exosomes are present in the PF and their role in epicardial stem cell-mediated cardiac repair. Methods and Results: Exosomes were identified in PFs from non-infarcted patients (PFC) and patients with acute myocardial infarction (PFMI). A shotgun proteomics analysis of exosomes from PFC and PFMI was used to identify differentially expressed proteins in these microvescicles. We identified Clusterin in exosomes isolated from PFMI but not from PFC. Notably, clusterin is an important mediator of TGF β -induced epithelial-to-mesenchymal transition (EMT), a key process which regulates epicardial cell activation and differentiation. Both q-RTPCR and immunohistochemical studies showed cardiomyocytes expressing clusterin in the infarcted region 3 days following MI. The importance of EMT in PF-induced epicardial cells was confirmed by gene expressions studies on epicardial cells from infarcted mouse hearts in the presence and in the absence of PF. Three days following MI, the expression of genes related to EMT, i.e. Snai1 and Twist1 were increased in the presence of PF. Noteworthing, EMT genes were specifically upregulated in epicardial cells expressing the stem cell antigen c-kit as demonstrated by in vitro studies. In vivo studies are ongoing to demonstrate the involvement of clusterin in the PFMI induction of EMT and myocardial repair. Conclusions: Exosomes are present in the PFs. Clusterin was identified only in PFMI and may account, at least in part, for epicardial EMT and myocardial regeneration following MI.
Biography
Federica Limana is working as a Post-doctoral fellow in the laboratory of Prof. Matteo A Russo at the University of Roma ?Sapienza? (Dipartimento di Medicina Sperimentale). Her studies have led to several publications on aspects of cardiac regeneration in the injured heart. Specifically, in the last few years she studied the role of the adult epicardium in the physiologic process of myocardial regeneration and identified in this compartment a pool of cells that may represent a new source of CSCs
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version