alexa Role Of Transient Receptor Potential Canonical 3 Channel (TRPC3) And Polyphenols On Manganeseinduced Toxicity Of Hippocampal Astrocytes | 70935
ISSN: 2155-9562

Journal of Neurology & Neurophysiology
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

14th World Congress on Neurology and Neurological Disorders

Annie Phung and Harold Komiskey
Philadelphia College of Osteopathic Medicine-Georgia Campus, USA
Posters & Accepted Abstracts: J Neurol Neurophysiol
DOI: 10.4172/2155-9562-C1-056
Abstract
Introduction & Aim: Polyphenols, Epigallocatechin (EGC), Epigallocatechin-3-Gallate (EGCG) and Myricetin (MRC) are involved in natural preventative care. However, in metal neurotoxicity, these mechanisms are not clearly identified. Manganese (Mn) is a trace element but is toxic at higher concentrations from occupational and/or environmental exposures. Astrocytes play a vital role in neuronal signaling. When exposed to toxins, astrocytes undergo astrogliosis, characterized by increased production of glial fibrillary acidic protein (GFAP). Transient Receptor Potential Canonical 3 channel (TRPC3) is known to influence Ca flux of cortical astrocytes, necessary for signaling. However, Mn has been shown to block Ca flux by TRPC3. In this study, we have revealed the presence of TRPC3 on hippocampal astrocytes and the effect of polyphenols on TRPC3 regulation, GFAP production and viability of Mn-induced astrocyte toxicity. Methods: Astrocytes (E18) were isolated from hippocampal tissue and cultured for 12 days. Cells were plated with 1, 3 and 10 uM of EGC, EGCG and MRC, incubated for 24 hours. Mn (100 uM) was added to wells and incubated for 48 hours. WST-8 detected cell viability and immunofluorescence identified GFAP and TRPC3. Results: Immunofluorescence indicated the presence of TRPC3 channel on hippocampal astrocytes. WST-8 showed consistent decreased viability when Mn was added to cells. Increased GFAP confirmed that Mn caused damage to astrocytes. EGC (3 uM), EGCG (1 uM), MRC (1 uM) appeared to decrease GFAP and TRPC3. Conclusion: TRPC3 is present on hippocampal astrocytes. Certain concentrations of polyphenols may be astroprotective against Mn-induced damage. Discussion: For the first time, TRPC3 channel was demonstrated on hippocampal astrocytes. The regulation of TRPC3 in Mn toxicity is not clear, though literature suggests that the channel is up-regulated because astrocytic Ca signals are needed to initiate astrogliosis. EGC (10 uM) and MRC (3 uM) may prevent damage produced by Mn.
Biography

Annie Phung is currently a Master’s degree candidate at the Philadelphia College of Osteopathic Medicine - Georgia Campus, USA. She has completed her undergraduate degree in Biological Sciences at the University of California, Irvine in 2015 where her knowledge about neuroscience research started in the lab of Dr. Burton-Jones.

Email: [email protected]

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7