alexa RTVP-1 Promotes The Mesenchymal Transformation Of Glioma Stem Cells Via The CXCR4 And The IL-6 Pathways
ISSN: 1948-5956

Journal of Cancer Science & Therapy
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

4th World Congress on Cancer Science & Therapy
October 20-22, 2014 DoubleTree by Hilton Hotel Chicago-North Shore Conference Center, USA

Chaya Brodie, Hae Kyung Lee, Nis David Giladi, Susan Finniss, Simona Cazacu, Cunli Xiang, Laila Poisson and Amotz Ziv-Av
Accepted Abstracts: J Cancer Sci Ther
DOI: 10.4172/1948-5956.S1.035
Abstract
Glioblastoma, the most aggressive primary brain tumors, are categorized into the major subgroups: Proneural, neural, classical and mesenchymal, the latter being characterized by increased invasion and poor prognosis. We recently identified RTVP-1 as a glioma-associated protein that regulates glioma cell migration and invasion. Using ChiP analyses, we found that the RTVP-1 promoter bind STAT3 and C/EBPbeta. Analysis of TCGA tumor specimens demonstrated that the expression of RTVP-1 was higher in the mesenchymal GBM and was inversely correlated with patient survival. We further found that RTVP-1 was expressed in glioma stem cells (GSCs) but not in human neural stem cells (NSCs). Overexpression of RTVP-1 in NSCs induced their mesenchymal transformation, whereas silencing of RTVP-1 in GSCs decreased their mesenchymal signature, increased their neural phenotypes and inhibited their self renewal and stemness. Silencing of RTVP-1 also decreased tumor volume of GSC-derived xenografts and increased animal survival. Using gene array analysis of RTVP-1 silenced cells we identified IL-6 and CXCR4 as major mediators of RTVP-1 effects on the mesenchymal transformation and self-renewal of GSCs. Using a pull down assay with His-tagged RTVP-1 and FRET analysis, we identified HSP27, N-WASP and hnRNPK as novel interacting proteins of RTVP-1, that mediate its effects on GSC migration and invadopodia formation. In summary, RTVP-1 promotes the mesenchymal transformation of GSCs and induces self-renewal and migration by the increased expression of IL-6 and CXCR4 and via its interaction with N-WASP, hnRNPK and HSP27. Collectively, these results implicate RTVP-1 as a novel prognostic marker and therapeutic target in GBM.
Biography
Chaya Brodie has completed her PhD at Bar-Ilan University, Israel and postdoctoral studies at University of Colorado and National Jewish Center for Immunology and Respiratory Medicine. She is a Senior Staff Scientist and the Director of Translational Science in the Hermelin Brain Tumor Center, Henry Ford Hospital, Detroi, MI and a Professor in the Faculty of Life Sciences, Bar-Ilan University, Israel. She has published more than 135 papers in reputed journals and has served as a reviewer in various NIH study sections and in international foundations and societies.
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords