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|Wuhan Institute of Virology-Chinese Academy of Sciences, China|
|ScientificTracks Abstracts: Dentistry|
|Dental caries remains one of the most common global chronic diseases caused by Streptococcus mutans, which is prevalent all over the world. A potent and effective anti-caries vaccine has long been expected for caries prevention but no vaccines have been brought to market till now mainly due to the low ability to induce and maintain protective antibody in oral fluids. We explored different fusion strategies for the generation of a single fusion protein composed of both caries vaccine antigen and flagellin. We found that intranasal immunization with a single recombinant fusion protein of flagellin and PAc (KF-rPAc) can induce PAc-specific salivary IgA antibody responses and endow protection against caries. Optimization of the fusion strategy by replacement of the highly variable region of flagellin with caries vaccine antigen could increase specific IgA antibody response and protection efficacy. Novel recombinant fusion proteins KFD2-rPAc in which the hyper-variable region of flagellin (KF) was replaced with PAc could induce robust PAc-specific serum IgG and IgA as well as PAc-specific salivary IgA, meanwhile lower the antibody response against KF compared with KF-rPAc. More importantly, KFD2-rPAc could provide higher protective efficacy than that by KF-rPAc, but with limited anti-body response not needed for protection against caries. KFD2-rPAc can be a superior caries vaccine candidate for possible clinical trial. In conclusion, the fusion protein composed of both caries vaccine antigen and flagellin is an effective strategy to enhance salivary IgA response and anti-caries efficacy for development of caries mucosal vaccine.|
Huimin Yan has completed his BS and PhD from Wuhan University, China. He has completed his Post-doctoral studies as a Research Associate in the Department of Pathology, School of Medicine, Case Western Reserve University, USA on Mucosal Immunology. He is a Professor in Wuhan Institute of Virology, CAS and PI of Mucosal Immunity Research Group.
Email: [email protected]
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