Sao Paulo Workers Exposed To Air Pollution: Evaluation Of Genotoxicity | 35897
Journal of Molecular Biomarkers & Diagnosis
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The goal of this study was to evaluate the genotoxic effects of air pollution (PM2.5) on exposed individuals, from Sao Paulo City.
The study involved 58 male workers of two different exposition groups: 1) 26 taxi drivers (TD) and 17 traffic controllers (TC),
high exposure and 2) 15 workers from the Forest Institute (FI), low exposure. Each voluntary used a Personal Environmental
Monitoring Sampler to collect PM2.5 particles mass. The sampler were operated continuously at 4-LPM over 24-hours. Particles were
collected on a polycarbonate membrane filter (37 mm diameter). The frequency of Micronuclei (MN) in buccal exfoliated cells and
peripheral blood lymphocytes were determined. The average the MN in lymphocytes and buccal exfoliated cells was significantly
higher on group 1 (PM2.5 p < 0.001). There is a significant correlation between MN measured in exfoliated cells and in lymphocytes
(correlation coefficient of 0.80; p<0.001). So the determination of MN buccal exfoliated cells is as efficient as the determination of
MN in lymphocytes, a classic biomarker for the assessment of genotoxicity. Our results have shown that individuals exposed to the
highest concentrations of PM2.5 have a higher level of micronucleus. However, we found in our study some individuals that even
exposed to lower concentrations of pollutants, showed higher levels of micronuclei which allowed us to identify them as individuals
more susceptible to PM2.5. Thus, in order to understand this susceptibility, the methylation study of genes promoters related to
inflammatory response, will be carried trough in these individuals.
Mônica Valeria Marquezini is a Scientific Researcher at Pro-Blood Foundation of Blood Center of São Paulo since 1991. She is a part of Experimental Air Pollution Laboratory - LPAE staff at the Department of Pathology, Medical School of University of São Paulo, Brazil, since 2010. She is an expert in Cellular and Molecular Biology with cell surface proteins studies and extracellular matrix research. She is a Teacher of Cell and Molecular Biology themes at different graduation careers, Post-graduation advisor, and Coordinator of specialization courses and National and International Projects. She holds a PhD in Science and from the Federal University of São Paulo-School of Medicine (1996) and graduated in Biological Sciences Medical Modality (1983).