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Screening of indigenous environmental bacteria for production of bioactive compounds (BaCs) and their genes
World Congress and Expo on Applied Microbiology
August 18-20, 2015 Frankfurt, Germany

Nadia Jamil1,3, Nuzhat Ahmed2 and Hartmut Laatsch3

1University of Karachi, Pakistan 2National Institute of Blood Diseases and Bone marrow Transplantation, Pakistan 3Der-George August University, Germany

Posters-Accepted Abstracts: J Microb Biochem Technol

Abstract:

Our environment is a rich source of microorganisms �?nclud�?ng bacteria and fungi that can produce s�?gn�?ficant bioactive compounds (BaCs). These microbes have been shaping their environment and thus the earth�??s b�?osphere for b�?ll�?ons of years through an enormous range of BaCs. These days, as a result of far-reach�?ng research, the study of m�?crob�?al BaCs �?s recogn�?zed as an essent�?al component of natural products chem�?stry and as well as cons�?derable env�?ronment fr�?endly compounds, wh�?ch have var�?ous �?ndustr�?al and agr�?cultural appl�?cat�?ons. �?n Pak�?stan the knowledge regard�?ng the �?nd�?genous m�?crobes capable of produc�?ng BaCs is in very early stages. Our purpose of this study was to screen two microbes CMGN122 and CMGN370 isolated from Sindh, Pakistan for production of BaCs and their genes. Biological screening samples from microbial fermentation extracts were obtained after optimizing growth conditions and extraction procedures that capture BaCs produced. Column chromatography (CC) and TLC were used for isolation and compound identification and, structure elucidation was done by different techniques of NMR and Mass Spectrometry. CMGN122 identified as Pseudomonas aeruginosa (GenBank Accession No. JN969597) was found to produce three different Phenazine compounds and pyrroloquinoline quinone (Pqq). CMGN370 identified as Candida sp. (GenBank Accession No. JN969598) showed production of two compounds Daidzein and N-(5-Acetylamino-pentyl)-acetamide. Genes for phenazines and PqqC were isolated and amplified.