Electrical cell-substrate impedance sensing (ECIS) technique can be used to detect cell behaviors, such as cell adhesion, cell
growth, cell apoptosis and cell metabolism, on a substrate with electrodes. In this study, aiming to monitor cell behaviors
in real time, we developed screen-printed interdigitated electrodes with electrode widths and spacings commensurate with
cell sizes. In general, screen printing technique provides advantages in fabricating costs, convenience in design and thick film
characteristic. However, the difficulty in miniaturizing the electrode feature size has limited the adoption of screen printing in
some applications. By using appropriate steel plate and silver ink in screen printing fabrication, we have succeeded in scaling
down the electrode width and spacing to sub 100μm. In this study, electrodes with different spacings and widths were designed
to evaluate their sensitivity of detecting cell adhesion. Our experiment results showed that reducing the electrode dimension can
increase the cell adhesion sensitivity, supposedly because of more concentrated electric field distribution surfacing the substrate
and electrodes. Finally, the optimized electrode width and spacing for cell detection were decided and ECIS measurements were
conducted. This interdigitated electrode design could be applied in cell biology researches in the future.
Y.J. Lin is a MS student of Electrical Engineering in National Central University, Jung-Li City, Taiwan. He received the BS degree in electrical
engineering from Chang Gung University, Taoyuan City, Taiwan. His current research interests include biomedical signal processing, biosensor,
micro-processor and C and Matlab programing.
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