Secondary metabolites of methanolic extracts from chilca (Baccharis glutinosa) roots against phytopathogenic fungi

LamGutierrez A MontesMolina J AyoraTalavera T GutierrezMiceli F GarridoRamirez E; RuizValdiviezo Victor

doi:10.4172/2161-0495-C1-025

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9^th Euro-Global Summit on Toxicology and Applied Pharmacology
June 22-24, 2017 Paris, France

Lam-Gutierrez A, Montes-Molina J, Ayora-Talavera T, Gutierrez-Miceli F, Garrido-Ramirez E and Ruiz-Valdiviezo Victor

Tuxtla Gutierrez Institute of Technology, México

Posters & Accepted Abstracts: J Clin Toxicol

DOI: 10.4172/2161-0495-C1-025

Abstract

Baccharis glutinosa has been used in Mexico in anti-fungal activity on maize phytopathogenic fungi; the aerial parts have been tested producing a fungistatic effect against Aspergillus flavus, A. parasiticus and a fungicidal effect on F. verticillioides. Also, it suggest that the antifungal fractions act producing a defective cell wall, the fractions act as competitive inhibitor of the enzyme β-1,3 glucanase. In this study, we investigated the inhibitory effect of methanol extract obtained by the crude extract of roots, on two major fungal pathogens of agricultural impact. The antifungal activity was evaluated by minimum inhibitory concentration and minimum fungicidal concentration methods against Aspergillus ochraceus Wilhelm and Fusarium moniliforme J. Shield, using different concentrations of methanolic extract: 0.09, 0.16, 0.26, 0.43, 0.73, 1.21, 2, 3.4, 5.6, 8, 10, 12 and more that 15 mg.mL-1. Ketoconazole (1 mg.mL-1) was used as control. The treatments were applied in triplicate. These results demonstrate that the use of methanolic extracts chilca have an effect on the sporulation inhibition of Aspergillus ochraceus Wilhelm and Fusarium moniliforme J. Shield with increasing concentrations of study. The methanolic extracts had inhibitory effect on the fungal Aspergillus ochraceus Wilhelm and Fusarium moniliforme J. Shield. Preliminary studies in GCMS analysis of methanolic extracts of chilca revealed the presence of furfural compounds and organic acids. To our knowledge, this is the first study reporting of phytochemical composition and biological activity of Baccharis glutinosa roots that could be used as natural alternative to biological control of pathogenic fungi.

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Journal of Clinical Toxicology
Open Access