Pharmaceutica Analytica Acta

Sensitive and rapid HPLC-UV method with back-extraction step for the determination of sildenafil in human plasma

International Conference and Exhibition on Advances in HPLC & Chromatography Techniques

March 14-15, 2016 London, UK

Bayan Alkhawaja, Hamza Al-Hroub, Eman Alkhawaja and Tawfiq Arafat

Faculty of Pharmacy & Medical Sciences, Petra University, Amman-Jordan

Scientific Tracks Abstracts: Pharm Anal Acta

Abstract:

In this work we provided a selective, sensitive and rapid HPLC-UV method for quantification of sildenafil in human plasma. We have adopted a simple liquid�??liquid extraction procedure followed a back extraction in 5% perchloric acid solution. Chromatographic separation was achieved on a BDS C-18 Column (150 mm �? 4.6 mm, 5 m) using a mobile phase consisted 63% water, 37% acetonitrile and 0.1% triethylamine (pH 7.7). The analysis was detected at 230 nm. The achieved lower limit of quantification was 2.00 ng/ml. The method showed linear calibration curve over the range of 2.00�??200 ng/ml. Intra- and inter day precision (CV%) were less than 6.80 and 5.19%, respectively. Whilst intra- and inter day accuracy% were ranged between (98.3 and 105%) and (99.4 and 103%), respectively. Tests confirmed the stability of sildenafil in plasma at room temperature for 24 h, during three freeze�??thaw cycles, after 24 h in autosampler at 10°C and after 60 days in plasma at �??30°C. The recovery of sildenafil was greater than 78.4%. The described simple UV method achieved very low limit of quantification and by using simple and inexpensive extraction procedure, complete separation was obtained within short run time. Having demonstrated the validity and novelty of our method, thus it is applicable for the clinical and pharmacokinetic studies of sildenafil in human volunteers especially in laboratories in countries where cost of modern techniques and instrumentation is prohibitive.

Biography :

Bayan Alkhawaja completed her Master’s degree in Pharmaceutical Sciences, with cumulative average of 4 out of 4, last year from Petra University, Amman- Jordan. Currently she is pursuing her PhD at Department of Pharmacy and Pharmacology at University of Bath. She worked as a Pharmacist and then as Research Assistant at University of Petra.

Email: ph-bayan@hotmail.com