This study was conducted to investigate serum proteome alteration in falciparum (FM) (n = 20) and vivax malaria (VM) (n
= 17) patients from a malaria endemic area of India to obtain mechanistic insight about the disease pathogenesis and host
immune response using multiple proteomic techniques. The eventual goal of this study was to identify potential markers that
can distinguish the malaria patients from healthy controls (HC) as well as discriminate between FM and VM with high accuracy.
Our results revealed differential expression of 63 and 67 (p < 0.05) serum proteins in FM and VM, respectively, compared to
HC, nearly half of which (46.2%) were commonly modulated due to both plasmodial infections. Several identified proteins were
found to be involved in different vital physiological pathways, including acute phase response signaling, chemokine and cytokine
signaling, complement and coagulation cascades. A sub-set of the identified proteins was used to build statistical sample class
prediction models employing PLSDA and other classification methods for FM, VM and HC discrimination. For the first time we
have demonstrated the discrimination between falciparum and vivax malaria patients based on protein expression in host. This
study will enhance our understanding about the basis of the disease pathogenesis and host immunological responses triggered
by these parasitic infections. Furthermore, some of the identified differentially expressed proteins such as haptoglobin, serum
amyloid A, hemopexin, and complement C4 could further be studied as potential candidate diagnostic and/or prognostic serum
biomarkers for malaria.
Sanjeeva Srivastava is Group Leader of Proteomics Laboratory at IIT Bombay India. He obtained his Ph.D. from the University of Alberta and
post-doc from Harvard Medical School. Current research in this group centers on using HT proteomics for biomarker discovery in malaria and
other diseases, studying protein interactions and drug target discovery. He is recipient of several awards including National Young Scientist Award
(Canada), Young Scientist Awards (India) and the Apple Research Technology Support Award (UK). He serves as Editor-in-Chief for International
Journal of Genomics and Proteomics, and Associate Editor for several international journals, including Journal of Integrated OMICS.
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