alexa Sevoflurane Pretreatment Reduces High Glucose-induced Oxidative Stress And Inflammatory Injury In Human Umbilical Vein Endothelial Cells
ISSN: 2155-6156

Journal of Diabetes & Metabolism
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading Please wait..

5th World Congress on Diabetes & Metabolism
November 03-05, 2014 Embassy Suites Las Vegas, USA

Suobei Li
ScientificTracks Abstracts: J Diabetes Metab
DOI: 10.4172/2155-6156.S1.026
Background: Hyperglycemia induced oxidative stress and inflammatory injury result in endothelial dysfunction, which is a risk factor for cardiovascular disease. Sevoflurane, one of the most commonly used volatile anesthetics, has been proven to be effective in combating oxidative stress and protecting organs against inflammatory injury in various conditions. We hypothesized that sevoflurane pretreatment could protect endothelial cells against high glucose-induced endothelial dysfunction, and that the protective effects of sevoflurane preconditioning might be associated with the reduction of inflammatory cytokines. Methods: Primary cultured human umbilical vein endothelial cells (HUVECs) were exposed to sevoflurane (0.5, 1.5 and 2.5 minimum alveolar concentration, MAC) for 30 min with or without N(G)-nitro-L-arginine methyl ester (L-NAME, a nitric oxide synthase inhibitor) 1 mMfor 1 hour, and then incubated with high glucose (HG, 25 mM) for 12 hours, respectively. Tumor necrosis factor-α(TNF-α) release in culture medium was detected by using ELISA. Expression of intracellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) (cell ELISA and Western blot) and activation of NF- κB (Western blot) were also assessed. Adhesion of HRP-labeledHL-60 to HUVECs was measured by a spectrophotometer. The intracellular reactive oxygen species (ROS) production was assessed by monitoring the DCF fluorescence level after incubation with 25 mM glucose for 1 hour. Results: In our study, the high glucose-induced increases in TNF-α release and expression of ICAM-1 and VCAM-1 were significantly attenuated by pretreatment with sevoflurane in a dose-dependent manner in cultured HUVECs. Enhanced cell adhesion caused by high glucose in co-cultured HL-60 and HUVEC was also blocked by pretreatment with sevoflurane. Pretreatment with sevoflurane also blocked formation of high glucose-induced ROS in HUVECs. In addition, sevoflurane suppressed the transcriptional activity of NF-κB and IκB phosphorylation under high glucose conditions. Pretreatment with L-NAME attenuated the protective action of sevoflurane on high glucose-induced ICAM-1 and VCAM-1 expression, activation of NF-κB and leukocytes adhesion to HUVECsαsuggesting a potential role of nitric oxide (NO) signaling. Conclusion: The present data suggest that sevoflurane could suppress high glucose-induced oxidative stress and vascular inflammatory processes, and sevoflurane confers cytoprotective effects likely through inhibition of ROS and NF-κB activation in aNO-dependent manner in HUVECs.
image PDF   |   image HTML

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

1-702-714-7001Extn: 9037

Business & Management Journals


1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version