alexa Site Specific Expression Of The Wnt Signaling Target Gene Nkd1 In Mouse Intestine And Liver | 17436
ISSN: 2157-7013

Journal of Cell Science & Therapy
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

4th World Congress on Cell Science & Stem Cell Research

Stanč?kov? Jitka, Faf?lek Bohumil, Michaela Krausov?, Lucie Tůmov?, Hlavat? Ad?la and Koř?nek Vladim?r
Posters: J Cell Sci Ther
DOI: 10.4172/2157-7013.S1.029
Abstract
W nt signaling plays a crucial role in ontogenesis and development in all metazoans. In adult mammals, the Wnt signaling pathway is required for intestinal homeostasis and establishment of the hepatic zonation. In contrary, aberrant activation of the Wnt pathway leads to neoplasia and cancer development, notably in the intestine and liver. To investigate the role of the Wnt pathway in gut epithelium selfrenewal and malignant transformation we employed chromatin immunoprecipitation method (ChIP) in combination with DNA microarrays (so-called ChIP-on-chip) to identify genes regulated by Wnt signaling. One of the most prominent target was the NKD1 (Naked Cuticle Homolog 1) gene; previously identified as a Wnt-induced intracellular negative regulator of canonical Wnt signaling. We generated mice with CreERT2 recombinase produced from the Nkd1 locus (NKD1-CreERT2). Two of the most interesting tissues of the NKD1-CreERT2 expression in adult mice were pericentral hepatocytes and intestinal crypt compartments. In the small intestine NKD1-CreERT2 expression decreases in proximodistal manner with no expression in ileal stem cells. In the liver NKD1-CreERT2 displayed expression in pericentral hepatocytes where the Wnt signaling pathway is active. Gradient zonal expression of Nkd1 in the mouse intestine and liver gives insight into different regulation of Wnt signaling in these tissues
Biography
Jitka Stanč?kov? is a PhD student from Faculty of Science of Charles University in Prague. She works in Laboratory of Cell and Developmental Biology in Institute of Molecular Genetics in Academy of Science of the Czech Republic. Until today she published 4 papers in reputed journals
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

agriaq[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7