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To investigate the cellular change and cell death mechanism in cultured human lens
epithelial cells induced by smoking.Cultured lens epithelial cells were challenged with
200, 400, 600, 800, 1000 uM of Cadmium chloride(CdCl2, Catalog No. 202908,Sigma-
Aldrich Chemical Co, USA) for 2 hours, and then were exposed to UV(280-320nm/ 1.2mv/
cm2). The cell vialbility was evaluated using the microscpe and 3-(4,5-dimethylthiazol
-2-yl)-2,5-dipheny tetrazolium bromice (MTT) assay. The gene and protein level of
caspase-8 and P53 were measured after exposed to CdCl2(600uM) for 2 hours by using RT
PCR and western blot.Compared to untreated cell, the cell death increased after Cadmium chloride exposure in microscopic
finding. And MTT assay demonstrated that the cell death was increased in proportinal to the increased Cadmium chloride
concentration. The expression of caspase-8 and P53 level all increased after UV exposure in RT PCR and western blot.
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