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Smoking And Cataract (in Vitro 1) | 7988
ISSN: 2155-9570

Journal of Clinical & Experimental Ophthalmology
Open Access

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Smoking and Cataract (in vitro 1)

International Conference & Exhibition on Clinical Research Dermatology, Ophthalmology & Cardiology

Jae Woong Koh

ScientificTracks Abstracts: JCEO

DOI: 10.4172/2155-9570.S1.01

Abstract
To investigate the cellular change and cell death mechanism in cultured human lens epithelial cells induced by smoking.Cultured lens epithelial cells were challenged with 200, 400, 600, 800, 1000 uM of Cadmium chloride(CdCl2, Catalog No. 202908,Sigma- Aldrich Chemical Co, USA) for 2 hours, and then were exposed to UV(280-320nm/ 1.2mv/ cm2). The cell vialbility was evaluated using the microscpe and 3-(4,5-dimethylthiazol -2-yl)-2,5-dipheny tetrazolium bromice (MTT) assay. The gene and protein level of caspase-8 and P53 were measured after exposed to CdCl2(600uM) for 2 hours by using RT PCR and western blot.Compared to untreated cell, the cell death increased after Cadmium chloride exposure in microscopic finding. And MTT assay demonstrated that the cell death was increased in proportinal to the increased Cadmium chloride concentration. The expression of caspase-8 and P53 level all increased after UV exposure in RT PCR and western blot.
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