alexa Spatial And Temporal Expression Of Developmentally Regulated Proteins During Spinal Cord Formation | 64466
ISSN: 2329-6895

Journal of Neurological Disorders
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International Conference on Neurological Disorders & Stroke and Neurooncology

Shadid A, Al Dayel A, Fuller H R and Gates M A
Al Imam Mohammad Ibn Saud Islamic University, Saudi Arabia
Keele University, UK
Posters & Accepted Abstracts: J Neurol Disord
DOI: 10.4172/2329-6895-C1-018
Abstract
Background: Between 250,000 and 500,000 persons suffer a spinal cord injury every year. Previous proteomics analysis in the lab has identified proteins that are specifically expressed in the developing spinal cord of rats and may be important for the generation of the corticospinal tract (CST). Aim: The aim of this study was to characterize the timing and location of expression of these proteins in the developing rat to relate them to spinal cord formation. Methods: Cross sections from postnatal day (P) 0, 3, 7, 10, 14, 17 rat cervical spinal cord were incubated with antibodies against stathmin1 p38, DPYSL3, BLBP and CRABP1. Then, they were "stained" with an appropriate 594 (RED) fluorescent secondary antibody. Results: While the expression of CRABP1, phosphorylated stathmin 1 (p38) and DPYSL3 appear restricted to the CST at specific stages of development, BLBP appears to be more globally distributed throughout the spinal cord. Conclusion: This characterization offers insights into the role that certain developmentally regulated proteins may play in spinal cord formation and the potential importance of these proteins in future therapies for spinal cord injury.
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