alexa Structural Characterization And Biological Implications Of Sulfated N-glycans In A Serine Protease From The Neotropical Moth Hylesia Metabus (Cramer [1775]) (Lepidoptera: Saturniidae) | 67203
ISSN: 2168-958X

Journal of Glycobiology
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3rd Glycobiology World Congress
June 26-28, 2017 London, UK

Gleysin Cabrera, Victor Salazar, Raquel Montesino, Yanet Támbara, Weston B Struwe, Evelyn Leon, David J Harvey, Antoine Lesur, Mónica Rincon, Bruno Domon, Milagros Mendez, Madelon Portela, Annia Gonzalez-Hernández, Ada Triguero, Rosario Duran, Ulf Lundberg, Eva Vonasek and Luis Javier Gon
Center for Genetic Engineering and Biotechnology, Cuba
Venezuelan Institute for Scientific Research, Venezuela
Universidad de Concepción, Chile
University of Oxford, UK
Luxembourg Clinical Proteomics Center, Luxembourg
Unidad de Bioquímica y Proteómica Analíticas, Uruguay
Posters & Accepted Abstracts: J Glycobiol
DOI: 10.4172/2168-958X-C1-009
Abstract
Contact with the urticating setae from the abdomen of adult females of the neo-tropical moth Hylesia metabus gives rise to an urticating dermatitis, characterized by intense pruritus, generalized malaise and occasionally ocular lesions (lepidopterism). The setae contain a pro-inflammatory glycosylated protease homologous to other S1A serine proteases of insects. Deglycosylation with PNGase F in the presence of a buffer prepared with 40% H2 18O allowed the assignment of an N-glycosylation site. Five main paucimannosidic N-glycans were identified, three of which were exclusively α(1-6)-fucosylated at the proximal GlcNAc. A considerable portion of these N-glycans are anionic species sulfated on either the 4- or the 6-position of the α(1-6)-mannose residue of the core. The application of chemically and enzymatically modified variants of the toxin in an animal model in guinea pigs showed that the pro-inflammatory and immunological reactions, e.g., disseminated fibrin deposition and activation of neutrophils are due to the presence of sulfate-linked groups and not on disulfide bonds as demonstrated by the reduction and S-alkylation of the toxin. On the other hand, the hemorrhagic vascular lesions observed are attributed to the proteolytic activity of the toxin. Thus, N-glycan sulfation may constitute a defense mechanism against predators.
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