alexa Structural Insights Into The Elevator-like Mechanism Of The Sodium/citrate Symporter CitS | 72467
ISSN: 0974-276X

Journal of Proteomics & Bioinformatics
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

9th International Conference on Structural Biology
September 18-20, 2017 Zurich, Switzerland

Subin Kim, Ji Won Kim, Haerim Lee, Songwon Kim, Jie-Oh Lee and Mi Sun Jin
KAIST, Korea
SGIST, Korea
Posters & Accepted Abstracts: J Proteomics Bioinform
DOI: 10.4172/0974-276X-C1-101
Abstract
The 2-HCT family of transporters generally translocate molecules with a 2-hydroxycarboxylate motif (HO-CR1R2-COO-), such as citrate, malate and lactate across the plasma membrane, and activity is tightly coupled to energy from a sodium or proton gradient. Klebsiella pneumoniae CitS (KpCitS) is the best-characterized model system, which has been purified in detergent and characterized in a reconstituted state. It plays a key role for citrate uptake to ultimately produce ATP in anaerobic fermentative process. Single-molecule fluorescence spectroscopy study provided an evidence for formation of homodimeric KpCitS. Analysis of hydropathy profiles and rich biochemical data suggested that it consists of 11 transmembrane helixes with two putative reentrant loops. Mutational studies showed that R428, which is strictly conserved in transporters of the 2-HCT family, is critical for interaction with one of the carboxylate groups of citrate. Analysis of data from kinetics experiments demonstrated that KpCitS carries citrate followed by binding of sodium ion. However, there are conflicting data regarding exact stoichiometry. The structure of KpCitS was studied extensively by electron crystallography, providing a glimpse of its global structure. The crystal structure of a homologous symporter from Salmonella enterica (SeCitS) recently revealed that it forms an asymmetric dimer, and that each protomer embeds a substrate translocation pathway at the interface between the transport and the dimerization domains. That structure provided the first high resolution view of a member of the 2-HCT family; however, many details in the transport cycle remained unanswered.
Biography

Subin Kim graduated from Chonnam National University in 2014 and completed her MS from Gwangju Institute of Science and Technology (GIST) in 2016 and she joined as PhD candidate in School of Life Sciences at Gwangju Institute of Science and Technology (GIST) under Mi Sun Jin in 2016.

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version