Journal of Microbial & Biochemical Technology

Study of cylA and esp gene expression in Enterococcus faecalis culture in microfluidic conditions

World Congress and Expo on Applied Microbiology

August 18-20, 2015 Frankfurt, Germany

Fariba Gapeleh1, Mohammad Reza Mehrabi2, Mohsen Mirzaee2

1Borujerd Branch, Islamic Azad University, Borujerd, Iran 2Assistant Professor Department of Laboratory Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran

Posters-Accepted Abstracts: J Microb Biochem Technol

Abstract:

Background: Enterococcus faecalis normal intestinal flora of humans and one of the causes of nosocomial infections that cause urinary tract infections, endocarditis and menangeditise the ability to form biofilm on surfaces such as catheters, venous catheters artificial heart valves and ocular lenses, the ESP and cylA factors .The purpose of this study was to evaluate the ability of the bacteria in the biofilm formation and detection of virulence factors in clinical isolates of enterococci surface protein and cytolysin and Study of cyla and esp gene expression in Enterococcus faecalis culture in microfluidic conditions Material and methods: A total of 54 clinical E. faecalis isolates was collected from hospitals in Ahvaz Ability of biofilm formation was measured by Microtiter plate assay. All isolates were then examined for presence of the ESPand cylA genes . and Study of cyla and esp gene expression in Enterococcus faecalis culture in microfluidic conditions by RealTimePCR method. Results: The microtiter plate assay results showed that attachment abilities in 4 (7%) strains were strong, oundes.:in 10 (26%) strains were moderate, and in 14 (56%) strains were weak and 5 (11%) strains didn�??t form biofilm. The prevalence of the ESP and cylA genes identified by PCR among clinical isolated strains, and the results were 83% and 70.%, respectively. Gene expression in cultured microfluidic system compared with conventional cultivation increased in the laboratory Conclusion: Microfluidic system by controlling the hydrodynamic conditions, chemically stable gradiant can be effective in biofilm formation. And positive or negative have effects on biofilm bacteria and the expression of virulence genes. The pattern of gene expression different in environmental conditions. Increased expression of virulence genes in simulated body microfluidic system symptoms increase biofilm formation bacteria into the body, compared to what we are seeing in vitro.