Oral Health and Dental Management

Surface marker detection of adipose- derived stem cells using flow cytometry

4^th Asia Pacific Congress & Expo on Dental and Oral Health

July 27-29, 2015 Brisbane, Australia

Zahra Sarabadani

Posters-Accepted Abstracts: Oral Health Dent Manag

Abstract:

Recent evidence has shown that adipose tissue is the robust and most accessible source of mesenchymal stem cells. In this study ADSCs of intra scapular subcutaneous adipose tissue of rabbit had derived and cultured and the surface markers were detected via flow cytometry. In this study adipose tissue of intra scapular region of 12 male New Zealand rabbits was harvested during the direct surgery or liposuction. After stem cell preparation, they were evaluated in third passage for detecting the surface markers by flow cytometry. The results showed that ADSCs displayed identical level of 99.7%, 99.4%, 65.7% and 96.7% for CD 45, CD 90, CD 73 and CD44, respectively. Our results confirmed the possibility of obtaining ADSC from adipose tissue which is a very available tissue in the body which can be a great source for getting stem cells for regeneration applications in a near future.