alexa Synthesis And Characterisation Of Triethyl Ammonium BIS (aminobenzoato)phenylsilicate | 15358
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

International Expo and Conference on Analytrix & HPLC
October 22-24, 2012 DoubleTree by Hilton Chicago-North Shore, USA

Neena Garg
Accepted Abstracts: J Chromat Separation Techniq
DOI: 10.4172/2157-7064.S1.005
Abstract
C alcific aortic valve disease is the most common indication for valvular surgery in the United States. Cellular mechanisms are under intense investigation. This study hypothesizes that this disease develops secondary to a tissue stem cell niche. The niche is regulated by Wnt secretion from the endothelial layer to activate LRP5 receptor on the adjacent myofibroblast cell to form bone. Methods: Human ex vivo calcified valves versus control aortic valves were tested for Lrp5/Wnt3a expression by RTPCR, Western Blot and Immunohistochemistry. eNOS null mice: control (n=20), cholesterol (n=20), cholesterol + Atorvastatin (n=20), were tested for the development of aortic stenosis by Visual Sonics Echo, Immunohistochemistry for Wnt, Lrp5, Osteocalcin, PCNA and RTPCR for Lrp5 and Cbfa1. In vitro studies were performed to isolate Wnt3a from aortic valve endothelial cells in the presence of lipids with and without Atorvastatin using Anion exchange chromatography. Oxidative stress levels were tested via eNOS expression. Treated endothelial cell conditioned media with lipids with and without Atorvastatin was added to myofibroblast cells. Gene expression for Cbfa1, Lp5 and osteocalcin from the valve myofibroblast cells with the various treatments was measured by semi-quantitative RTPCR. Results: Secretion of Wnt3a(>300-fold,p<0.0001) from aortic valve endothelium in the presence of abnormal oxidative stress as measured by nitric oxide regulation and lipids as measured by eNOS enzymatic activity and tissue nitrite levels. Osteoblastogenesis in the adjacent myofibroblast cell treated with conditioned media by LRP5 receptor signaling. Human ex vivo calcified valves express LRP5 as compared to normal valves (p<0.0001).Cholesterol treated eNOS mice develop severe stenosis with an increase in Lrp5, Cbfa1, (3-fold increase(p<0.0001). Conclusion: Targeting the Wnt3a/Lrp5 pathway in valvular calcification presents a novel approach towards treating this disease. The Wnt3a/LRP5 cross talk mechanism is demonstrated in three models. These findings demonstrate important implications for the therapeutic regeneration of a normal valve.
image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version