Targeting EGFR For Fluorescence Optical Imaging Of Cancer | 1308
Journal of Cancer Science & Therapy
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Dysregulation of epidermal growth factor receptor (EGFR) is associated with many types of cancers. It is of great interest
to noninvasively image the EGFR expression
. Among different modalities, fluorescence optical imaging has the
advantage of low cost, easiness of handling and simplicity for multiplexing. Fluorescence in the near-infrared (NIR) spectral
region is especially desirable due to its reduced background and high penetration capability. Various EGFR-targeting molecules
have been studied for molecular imaging. These include antibodies, antibody fragments, natural ligand EGF, nanobody and
affibody. A couple of examples will be discussed, with the focus on IRDye?
800CW labeled EGF (EGF800) and EGFR-specific
Both EGF800 and Eaff800 were characterized for binding/uptake using EGFR-overexpressing cells. When used for
tumor imaging, the signal intensities of EGF800 had a good correlation with tumor sizes. In an orthotopic prostate tumor model,
the tumor growth was successfully tracked by EGF800.
imaging study of Eaff800 was conducted in A431 xenograft
tumors. The accumulation of EGF800 in the tumor could be identified 1 hr post-injection, and became most prominent after 1
d. The specificity of Eaff800 was confirmed by its high level of binding/uptake by A431 cells and low binding/uptake by HER2-
overexpressing cells. In combination with an HER2-specific probe Haff682, Eaff800 could be used to distinguish between
A431 (EGFR-overexpressing) and SKOV3 (HER2-overexpressing) tumors. Interestingly, the organ distribution pattern and
clearance rate of Eaff800 were different from those of Haff682. While Haff682 accumulated predominantly in the kidney, more
Eaff800 was found in the liver.
Haibiao (Herbert) Gong earned a Ph.D. degree in molecular biology at National University of Singapore. He did his postdoctoral research at
University of Pittsburgh, School of Pharmacy, and joined LI-COR Biosciences as a research scientist in 2007. His research focus at LI-COR is near-
infrared fluorescence imaging. He has published more than 30 papers in reputed journals.
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