Reach Us +44-1647-403003
Targeting Gene Expression In Endothelial Cells Of Specifi C Organs | 5231
ISSN: 2157-7013

Journal of Cell Science & Therapy
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Targeting gene expression in endothelial cells of specifi c organs

International Conference & Exhibition on Cell Science & Stem Cell Research

Nadia Jahroudi

ScientificTracks Abstracts: J Cell Sci Ther

DOI: 10.4172/2157-7013.S1.02

Background : Cyclin dependent kinase like 1 (CDKL1) is a member of cell division control protein 2 (CDC2), plays an important impact on the progress. Th is study aimed to evaluate the related serine/threonine protein kinases family, and it is likely to occur in malignant tumors expression of CDKL1 in breast cancer and regulation in cancer cell growth. Methods: We investigated both the CDKL1 mRNA level in fresh biopsy tissues from 62 breast cancer patients and 20 benign tissues by real-time PCR, and CDKL1 protein in 20 paraffi n-embedded tissues from primary breast cancer patients by immunohistochemistry. Th e roles of CDKL1 in cell growth were analyzed with CDKL1 shRNA inhibitor-transfected cells. Results: CDKL1 was overexpressed in patients with breast cancer. CDKL1 provided a positive detection effi ciency of 89%, which was signifi cant higher than that of 44% by estrogen receptor and 27% by progesterone receptor (P<0.001). CDKL1 shRNA inhibitor-transfected cells exhibited obvious accumulation at G2/M phase and reduced cell growth when treated with the chemotherapeutic drugs. Conclusion: Both the CDKL1 level and its behavior in shRNA interference suggested that CDKL1 could be potentially developed as a tumor assistant marker for diagnosis and as a potential therapeutic target for breast cancer
Relevant Topics