Cyclin dependent kinase like 1 (CDKL1) is a member of cell division control
protein 2 (CDC2), plays an important impact on the progress. Th
is study aimed to evaluate the
related serine/threonine protein kinases family, and it is likely to occur in malignant tumors
expression of CDKL1 in breast cancer and regulation in cancer cell growth.
We investigated both the CDKL1 mRNA level in fresh biopsy tissues from
62 breast cancer patients and 20 benign tissues by real-time PCR, and CDKL1 protein in 20
n-embedded tissues from primary breast cancer patients by immunohistochemistry. Th
roles of CDKL1 in cell growth were analyzed with CDKL1 shRNA inhibitor-transfected cells.
CDKL1 was overexpressed in patients with breast cancer. CDKL1 provided a
positive detection effi
ciency of 89%, which was signifi
cant higher than that of 44% by estrogen
receptor and 27% by progesterone receptor (P<0.001). CDKL1 shRNA inhibitor-transfected
cells exhibited obvious accumulation at G2/M phase and reduced cell growth when treated with
the chemotherapeutic drugs.
Both the CDKL1 level and its behavior in shRNA interference suggested
that CDKL1 could be potentially developed as a tumor assistant marker for diagnosis and as a
potential therapeutic target for breast cancer
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