Targeting transcription factor for treatment of HPV-Induced cervical cancer by herbal compounds

2^nd International Conference and Exhibition on Pharmaceutical Regulatory Affairs

November 23-24, 2012 Hyderabad International Convention Centre, India

Saurabh Maru, G Mugesh and B C Das

Accepted Abstracts: Pharmaceut Reg Affairs

Abstract :

Cervical cancer is the principal cause of cancer-related mortality in women of the developing countries that contribute more than 85% of global disease burden. Persistent infection with high-risk human papillomavirus (HR-HPV), most notably of the type 16 and 18, is an essential prerequisite for the development of cervical cancer resulting in dysregulation of host cell cycle by targeting pivotal cell cycle proteins p53 & pRb by their viral gene products E6 & E7, respectively. Constitutive expression of HR-HPV E6 and E7 oncogene is mainly dependent on the availability of host cell transcription factors that act upon viral promoter and enhancer region. Activator protein-1 (AP1), a heterodimer of a group of structurally and functionally related members of the Jun (c-Jun, JunB, JunD) and Fos family (c-Fos, FosB, Fra-1 and Fra-2) is one of the transcription factors that are essentially required for viral oncogene expression. The current work is about targeting the HPV oncogenic transcription on cervical cancer cells by herbal compounds. These compounds altered composition of transcription factor AP-1 from oncogenic to non oncogenic form thus downregulated constitutively active AP1 specific DNA binding activity and suppression of oncogenic c-Fos and cJun expression which was accompanied by inhibition of HPV18 transcription. In addition to inhibiting growth these compounds strongly induced apoptosis as evidenced by an increased expression of the pro-apoptotic protein Bax, suppression of the anti-apoptotic molecules Bcl-2, and activation of caspase-3 and cleavage of PARP-1.

Biography :

Mr. Saurabh Maru served as Scientist & Group Leader at CBST (a DST, India & UTC, France collaborative centre), VIT University, Vellore. Currently he is Assistant Professor at Shri G S Institute of Technology and Science, a premier Engineering and Science Institute of central India established in 1952. Mr. Saurabh Maru completed his Master of Pharmacy (Pharmacology) from RGUHS, Bangalore and secured 4th rank in state. He is doing his Ph.D. from Rajiv Gandhi Technical University, M.P. He has published a paper in BMC Complementary and Alternative Medicine and written a chapter in book ?Treatment of Advanced Stage Cancers: Current Status & Emerging Frontiers?. He delivered talk in many national and international conferences.