alexa Temperature Stress Mediated Oxidative And Antioxidant Defense In Withania Somnifera L. Dunal
ISSN: 2157-7471

Journal of Plant Pathology & Microbiology
Open Access

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2nd International Conference on Plant Science & Physiology
June 26-27, 2017 Bangkok, Thailand

Mamta Sharma and Sunil Puri
Shoolini University, India
Posters & Accepted Abstracts: J Plant Pathol Microbiol
DOI: 10.4172/2157-7471-C1-006
Withania somnifera, a multipurpose medicinal plant of the Himalayan region possesses antioxidant, antitumor, anti-inflammatory, antistress, immunomodulatory, hematopoetic, anti-ageing, anxiolytic, anti-depressive rejuvenating properties and influence various neurotransmitter receptors in the central nervous system. Withanolides, secondary metabolites present in W. somnifera, have neuron regenerative property. In order to evaluate heat stress mediated morphological, physiochemical, oxidative stress and antioxidant defence in the Withania somnifera, plant was subjected to varied temperature conditions. For temperature treatments the seedlings were exposed to five temperature conditions (8˚C, 18˚C, 38 ˚C, 48˚C and 58˚C). Seedlings grown at 22˚C were treated as control plants. Temperature treatments caused significant decrease in stem length, root length, fresh weight and dry weight in all the treatments. Changes in leaf area, membrane stability and relative water content were also observed and these protein, carotenoids, tocopherol, ascorbic acid and alkaloids decreased. The antioxidant enzymes like superoxide dismutase, catalase, peroxidase, ascorbate peroxidase, glutathione–S-transferase, DPPH (1, 1-diphenyl-2-picrylhydrazyl) and ABTS (2,2’-azino-bis-3ethyl benzthiazoline-6- sulphonic acid) enhanced due to stress treatments. Withanolides were found to be higher in high temperature treated plants. W. somnifera was found to have protective mechanism against oxidative damage by maintaining higher enzymatic and non-enzymatic antioxidants.

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