alexa Testicular Toxicity Characters And Mechanisms Of Hydroxyurea On Rats | 69248
ISSN: 2161-1459

Journal of Clinical & Experimental Pharmacology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

8th World Congress on Pharmacology and Toxicology
July 24-25, 2017 Melbourne, Australia

Han Yan, Li Zhou, Yong-wei Luo and Zu-yue Sun
Shanghai Institute of Planned Parenthood Research, China
Posters & Accepted Abstracts: Clin Exp Pharmacol
DOI: 10.4172/2161-1459-C1-020
Abstract
Objective: Apoptosis plays a dominant role in both spontaneous spermatogenesis and germ cell death. This study was aimed to investigate the functions of related genes in testicular germ cell death induced by Hydroxyurea (HU). Method: Wild-type (WT) and FasL Transgenic (TG) DBA/C57BL mice were intraperitoneal injected with 400 mg/kg HU. 12 hours later, testes were collected. Histomorphology of testis was observed by stained with Periodic Acid Schiff (PAS). Apoptosis was assessed by TUNEL assay. mRNA and protein levels of related genes were evaluated by quantitative RT-PCR and Western blot respectively. Results: The 2×2 factorial design comparative experiments between WT and TG mice showed that the TG mice exhibited a higher basal apoptotic index. The basal mRNA levels of Fas and FasL and protein levels of Fas, FasL, Caspase-3, Caspase-8 and Caspase-9 in TG mice were also higher than that in WT mice. 12 hours after injection of HU, the testicular tubules exhibited no significantly morphological changes but remarkably increased apoptosis index in both WT and TG mice, with the latter having the higher amplitude. Although HU up-regulated the mRNA of apoptosis related genes such as Fas and FasL in both TG and WT mice, the increased amplitude were more obvious in TG mice. By Western blot analysis, apoptosis related proteins Fas, FasL Caspase-3, Caspase- 8 and Caspase-9 were significantly increased in both WT and TG mice, with TG mice exhibiting a greater up-regulation. Conclusion: Germ cell apoptosis induced by HU treatment may be related to the FasL mediated signal transduction pathway.
Biography

Han Yan has completed the masters in Quality Assurance from National Evaluation Centre for the Toxicology of Fertility Regulating Drug. She has published more than 20 papers in China and abroad reputed journals.

Email: [email protected]

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version