The Antibacterial Activity Of Honey And Lemon Juice Against Streptococcus Pneumoniae And Streptococcus Pyogenes Isolates From Respiratory Tract Infections | 67635
Journal of Medical Microbiology & Diagnosis
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This study was aimed to determine the antibacterial activity of honey and/or lemon juice on strains of Streptococcus pneumoniae
and Streptococcus pyogenes from respiratory tract infections. Clinical isolates were collected from Ahmadu Bello University
Teaching Hospital (ABUTH), Zaria and Ahmadu Bello University Health Services (ABUHS) Samaru campus, Zaria. The isolates
were characterized by standard microbiological procedures. Antibacterial activities of the honey and lemon juice, as well as that of
some standard antibiotic formulations were assayed using agar well diffusion and broth dilution method. Minimum inhibitory and
bactericidal concentrations were carried out. Rate of kill was also carried out to determine the death/survival rate of the bacterial
isolates after exposure to the agents. Noticeable variations in the antibacterial activity of the agents were observed. Thus, inhibition
zones (mm) ranging from 10-22 (100% honey), 14-29 (100% lemon) and 20-29 (honey and lemon juice mixture) were obtained.
However, minimum inhibitory concentrations (μg/ml) range between 1.95-125 (ceftriaxone), 1.56-NI (gentamicin), 31.5-NI
(amoxicillin/clavulanic acid), 0.98-62.5 (levofloxacin), 50.0-NI (azithromycin), 20.0-75.0 (100% v/v honey), 22.5-47.5 (100% v/v
lemon juice) and 17.5-25.0 (honey and lemon juice mixture). However, for the rate of kill, honey and lemon juice mixture, lemon
juice affected complete killing at 120 minutes; while, ceftriaxone, levofloxacin and honey produced complete killing at 1440 minutes.
Therefore, from the findings, honey and lemon juice mixture, lemon juice, levofloxacin, ceftriaxone and gentamicin had higher
antibacterial activity than azithromycin, amoxicillin-clavulanic acid and honey. However, for the statistical analysis, at p≥0.05, there
is significant difference between honey and lemon juice mixture and honey. In conclusion, the bacterial isolates were more susceptible
to honey and lemon juice mixture, lemon juice, levofloxacin, ceftriaxone and gentamicin; but less susceptible to azithromycin,
amoxicillin-clavulanic acid and honey. Excellent bactericidal activity was observed with honey and lemon juice mixture, lemon juice
compared to the honey alone. The findings in this research therefore provides scientific basis to the use of honey and lemon juice as
an alternative medicine by the populace in the treatment of respiratory tract infections.