alexa
Reach Us +1-217-403-9671
The Bioavailability Of Heparin-binding Molecules On Vascular Walls Is Regulated By Heparan Sulfate And Flow Rate | 8406
ISSN: 0975-0851

Journal of Bioequivalence & Bioavailability
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

The bioavailability of heparin-binding molecules on vascular walls is regulated by heparan sulfate and flow rate

International Conference & Exhibition Bioequivalence and Bioavailability

Michael Fannon

ScientificTracks Abstracts: J Bioequiv Availab

DOI: 10.4172/0975-0851.1000001

Abstract
W e have been studying the bioavailability of heparin-binding molecules to vascular surfaces using a bioreactor that employs three-dimensional vessel architecture and pulsatile flow. This approach has been conducted in tandem with the design of a computer model to conduct simulations and make predictions of molecular interactions at the cell surface. We used a single pass experimental design to better measure the interactions in the microenvironment and used heparin-binding molecules as our model of binding to vascular surfaces. Heparan sulfate is on the cell surfaces of blood vessels and is involved in the capture and internalization of scores of molecules from angiogenic promoters, such as vascular endothelial growth factor (VEGF) to cholesterol-associated molecules such as low-density lipoprotein(LDL). Our experimental results, which were in close agreement with our simulations showed that expression of heparan sulfate is a crucial factor in the bioavailability of these molecules to cell surfaces. Substantial binding of molecules was measured with intact heparan sulfate. Enzymatic removal of heparan sulfate from the endothelial walls resulted in significantly decreased retention of all heparin-binding growth factors tested as well as LDL. Flow rates slightly above average capillary flow (0.6-3.0 ml/min) exercised strong regulation of capture. In all molecules tested, at any but the lowest flow rates, capture in a single pass was virtually ablated, suggesting that without a reasonable half-life there is little chance of capture on vascular surfaces with flow rates higher than capillary rates.
Biography
Relevant Topics
Top